Figure 1. Blood analysis and protein identification in zebrafish exposed to organochlorine pesticide mixtures at concentrations of 0.05, 0.25, 2.5 and 25 μg/L: (a) The fasting glucose level (×10 mg/dL, black) and fasting insulin level (ng/mL, gray) were described with the color difference and designed by *** p < 0.001 and ** p < 0.01; (b) The number of up- (black) and down-regulated (gray) proteins was shown.

Figure 2. Dynamics clustering analysis of proteins quantified at 0.05 μg/L organochlorine pesticide mixtures. (n = number of proteins included in each cluster).

Figure 3. (a) KEGG pathway-based enrichment analysis in clusters 1~6. The enrichment efficiency was evaluated by −log 10 of p values. (b) KEGG pathway-based enrichment analysis using proteins grouped in cluster 3. The Fisher’s exact test p value was used to evaluate the enrichment efficiency.

Figure 4. Validation of aldehyde dehydrogenase (ALDH2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression and proposed mechanism of OCPs-induced hyperglycemia in zebrafish: (a) Western blot analyses of ALDH2 and GAPDH expression at different concentrations of organochlorine pesticide mixtures; (b) A proposed pathway underlying hyperglycemia -related effects induced in zebrafish.