PUBLICATION
Type 2 Diabetes Induced by Changes in Proteomic Profiling of Zebrafish Chronically Exposed to a Mixture of Organochlorine Pesticides at Low Concentrations
- Authors
- Gao, Y., Lee, H., Lee, S., Kim, K.T.
- ID
- ZDB-PUB-220515-19
- Date
- 2022
- Source
- International Journal of Environmental Research and Public Health 19(9): (Journal)
- Registered Authors
- Keywords
- aldehyde dehydrogenase 2, glyceraldehyde 3-phosphate dehydrogenase, organochlorine pesticide mixtures, proteomics, type 2 diabetes mellitus, zebrafish
- MeSH Terms
-
- Animals
- Diabetes Mellitus, Type 2*/chemically induced
- Diabetes Mellitus, Type 2*/metabolism
- Female
- Glucose/metabolism
- Hydrocarbons, Chlorinated*/analysis
- Hydrocarbons, Chlorinated*/toxicity
- Pesticides*/analysis
- Proteomics
- Zebrafish/metabolism
- PubMed
- 35564385 Full text @ Int. J. Environ. Res. Public Health
Citation
Gao, Y., Lee, H., Lee, S., Kim, K.T. (2022) Type 2 Diabetes Induced by Changes in Proteomic Profiling of Zebrafish Chronically Exposed to a Mixture of Organochlorine Pesticides at Low Concentrations. International Journal of Environmental Research and Public Health. 19(9):.
Abstract
Effect of organochlorine pesticides (OCPs) mixtures on development of type 2 diabetes mellitus (T2DM) and the underlying mechanism, especially at protein levels, are largely unknown. We exposed a mixture of five OCPs to zebrafish at concentrations of 0, 0.05, 0.25, 2.5, and 25 μg/L for 12 weeks. Differentially expressed proteins (DEPs) were quantitatively identified in female zebrafish livers, and its functional study was conducted. The significantly high glucose and low insulin levels were observed only at 0.05 μg/L, linking to the different pattern of DEPs than other concentrations. A total of 1082 proteins was quantified, of which 321 proteins formed 6 clusters in protein dynamics analysis. The enriched pathways in cluster 3 showing distinct pattern of DEPs could explain the nonlinear response at 0.05 μg/L, indicating that OCP mixtures adversely affected proteins associated with mitochondrial function and energy metabolism. We proposed a feasible mechanism that decrease in expression of aldehyde dehydrogenase led to abnormal accumulation of aldehydes, reducing expression of glyceraldehyde 3-phosphate dehydrogenase, and resulting in disruption of glucose homeostasis. Our findings help to better understand the causality of T2DM by exposure to OCP mixtures and to identify biomarkers in the protein expression level.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping