- Title
-
Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
- Authors
- Lichtenegger, A., Mukherjee, P., Tamaoki, J., Bian, L., Zhu, L., El-Sadek, I.A., Makita, S., Leskovar, K., Kobayashi, M., Baumann, B., Yasuno, Y.
- Source
- Full text @ J. Biomed. Opt.
Imaging of 8-day-old zebrafish: (a1) scattering-based en face image; (a2) Sagittal B-scan image; (b1) birefringent en face image; (b2) birefringent sagittal tomogram; (c) coronal scatter intensity-based cross-section at position indicated in (a1) by a red dashed line; (d) volume rendering of the birefringence data; (e) white-light microscope image; and (f) composition image of the OCT scatter intensity (gray) and angiography (red) data. |
Imaging of 1-month-old zebrafish: (a) En face scattering-based image; (b1) transversal B-scan; (b2) birefringence tomogram; (c) composition image of the OCT scatter-intensity (gray) and angiography (red) data; (d1) transversal tomogram at the indicated position by red dashed lines in (a) with a zoom-in; (d2) birefringent-based B-scan; and (d3) DOPU tomogram (RPE, retinal pigment epithelium). |
Imaging of 2-month-old zebrafish: (a) 3D-rendering of the birefringence data; (b) transversal scattering-based tomogram at the indicated position by an orange dashed line in (a); (c1) transversal image in the tail region; (c2) birefringence image at the indicated position by a red dashed line; (d1) scattering-based B-scan image of the gills; and (d2) birefringence B-scan (Video 1, MP4, 8.5 MB [URL: https://doi.org/10.1117/1.JBO.27.1.016001.1] and Video 2, MP4, 14 MB [URL: https://doi.org/10.1117/1.JBO.27.1.016001.2]). |
Attenuation and birefringence analysis in various zebrafish organs: (a), (b) white-light photographs of 1- and 2-month-old zebrafish. Box-plot results of the attenuation coefficients and birefringence values in (c1), (c2) 1-month and in (d1), (d2) 2-month-old zebrafish. |