FIGURE SUMMARY
Title

Simultaneous Tests of Theaflavin-3,3'-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (Danio rerio)

Authors
Zhou, H., Wu, Y., Kim, E., Pan, H., He, P., Li, B., Chen, Y.C., Tu, Y.
Source
Full text @ Nutrients
Figure 1

The effect of TF3 on HepG2 cell viability (A) and cell morphology (B). Control, DMEM medium culture; TF3, DMEM medium containing different concentrations of TF3 (1, 5, 10, 20, 50, 100 μM) treating for 24 h. ** p < 0.01 versus control group.

Figure 2

TF3 treatment recovered glucose uptake in glucose-treated insulin resistance HepG2 cells. Control, DMEM medium culture; model, DMEM medium with 500 μM glucose treating for 24 h; TF3, DMEM medium containing different concentrations of TF3 (1, 5, 10, 20, 50 μM) treating for 24 h after glucose-treatment; Met, DMEM medium containing metformin hydrochloride (10 μM) treating for 24 h after glucose-treatment. * p < 0.05 versus model group., ## p < 0.01 versus control group.

Figure 3

TF3 treatment reduced glucose level in zebrafish. Results were expressed as the mean ± SD (n = 5). Control, water culture; model, water with 1 μM alloxan and 4% glucose culture for 24 h; TF3, water with different concentrations of TF3 (0.5, 2, 4, 6.7, 10, and 20 μg/mL) treating for 24 h after alloxan-treatment; Met, water with metformin hydrochloride (10 μg/mL) treating for 24 h after alloxan-treatment. ## p < 0.01 versus control group. ** p < 0.01 versus model group, *** p < 0.001 versus Model group.

Figure 4

TF3 inhibited alloxan-induced hyperglycemia via regulating PEPCK and GCK expression. (A) Blot results and (B) relative protein expression. Control, water culture; model, water with 1 μM alloxan and 4% glucose culture for 24 h; TF3, water with different concentrations of TF3 (2, 4, 10 μg/mL) treating for 24 h after alloxan-treatment; Met, water with metformin hydrochloride (10 μg/mL) treating for 24 h after alloxan-treatment.

Figure 5

TF3 promoted β cell regeneration from alloxan-induced damage in zebrafish. (A) Fluorescence phenotype of zebrafish β cells and (B) the fluorescence intensity of β cells. Data were presented as the mean ± SD. Control, water culture; model, water with 1 μM alloxan and 4% glucose culture for 24 h; TF3, water with different concentrations of TF3 (0.5, 2, 4, 6.7, 10, and 20 μg/mL) treating for 24 h after alloxan-treatment. ### p < 0.001 versus control group, ** p < 0.01 versus model group, *** p < 0.001 versus model group.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nutrients
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