PUBLICATION
Simultaneous Tests of Theaflavin-3,3'-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (Danio rerio)
- Authors
- Zhou, H., Wu, Y., Kim, E., Pan, H., He, P., Li, B., Chen, Y.C., Tu, Y.
- ID
- ZDB-PUB-211230-47
- Date
- 2021
- Source
- Nutrients 13(12): (Journal)
- Registered Authors
- Keywords
- HepG2 cells, anti-diabetic effect, diabetic zebrafish model, glucose metabolism enzymes, theaflavin-3,3′-digallate
- MeSH Terms
-
- Animals
- Biflavonoids/isolation & purification
- Biflavonoids/pharmacology*
- Catechin/analogs & derivatives*
- Catechin/isolation & purification
- Catechin/pharmacology
- Dose-Response Relationship, Drug
- Down-Regulation/drug effects
- Drug Evaluation, Preclinical/methods*
- Glucokinase/metabolism
- Glucose/metabolism
- Hep G2 Cells
- Humans
- Hypoglycemic Agents*
- Phosphoenolpyruvate Carboxykinase (ATP)/metabolism
- Tea/chemistry
- Up-Regulation/drug effects
- Zebrafish
- PubMed
- 34959930 Full text @ Nutrients
Citation
Zhou, H., Wu, Y., Kim, E., Pan, H., He, P., Li, B., Chen, Y.C., Tu, Y. (2021) Simultaneous Tests of Theaflavin-3,3'-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (Danio rerio). Nutrients. 13(12):.
Abstract
Theaflavin-3,3'-digallate (TF3) is the most important theaflavin monomer in black tea. TF3 was proved to reduce blood glucose level in mice and rats. However, the elaborate anti-diabetic mechanism was not well elucidated. In this work, human hepatoma G2 (HepG2) cells and zebrafish (Danio rerio) were used simultaneously to reveal anti-diabetic effect of TF3. The results showed that TF3 could effectively rise glucose absorption capacity in insulin-resistant HepG2 cells and regulate glucose level in diabetic zebrafish. The hypoglycemic effect was mediated through down-regulating phosphoenolpyruvate carboxykinase and up-regulating glucokinase. More importantly, TF3 could significantly improve β cells regeneration in diabetic zebrafish at low concentrations (5 μg/mL and 10 μg/mL), which meant TF3 had a strong anti-diabetic effect. Obviously, this work provided the potential benefit of TF3 on hypoglycemic effect, regulating glucose metabolism enzymes, and protecting β cells. TF3 might be a promising agent for combating diabetes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping