FIGURE SUMMARY
Title

Toxicity of Jegosaponins A and B from Styrax japonica Siebold et al. Zuccarini in Prostate Cancer Cells and Zebrafish Embryos Resulting from Increased Membrane Permeability

Authors
Nishimura, M., Fuchino, H., Takayanagi, K., Kawakami, H., Nakayama, H., Kawahara, N., Shimada, Y.
Source
Full text @ Int. J. Mol. Sci.
Figure 1

Jegosaponin A and B from unripe fruits of Styrax japonica Siebold et al. Zuccarini (SJSZ) suppress prostate cancer PC-3 cells. (a) BuOH extract of SJSZ suppresses PC-3 cell viability. n = 8, error bars indicate SD. **** p < 0.0001 compared to control (0.1% DMSO). (b) Fraction 1, 5 and 6 of BuOH extract of SJSZ suppresses cell viability in a dose-dependent manner. n = 8, error bars indicate SD. **** p < 0.0001 compared to control (0.1% DMSO). (c) Chemical structures of jegosaponin A and B. (d) Jegosaponin A and B suppress PC-3 cell viability after 48 h treatment. n = 8, error bars indicate SD. **** p < 0.0001 compared to control (0 μM jegosaponin A). #### p < 0.0001 vs. control (0 μM jegosaponin B).

Figure 2

Zebrafish embryo acute toxicity test of jegosaponin A and B. (a) Blastula (5 hpf) embryos were exposed to jegosaponin A and B for 120 hpf. n = 3, * p < 0.05 and ** p < 0.01 vs. control (0 μM jegosaponin A); ## p < 0.01 vs. control (0 μM jegosaponin B) (b) Survival analysis on 24 hpf with jegosaponins exposure. * p < 0.05 and ** p < 0.01 vs. control (0 μM jegosaponin A); ## p < 0.01 vs. control (0 μM jegosaponin B). (c) Representative images of (a).

Figure 3

Jegosaponin A and B increase cell membrane permeability. (a) Haemolysis of RBCs by jegosaponin A and B. n = 3, error bars indicate SD. (b) Increase in cell membrane permeability of PC-3 cells treated with jegosaponin A and B. Purple indicates membrane impermeable DRAQ7-stained nucleus. (c) Quantitative analysis of (b). n = 3, error bars indicate SD. * p < 0.05 and ** p < 0.01 vs. control (0 μM jegosaponin A); # p < 0.05 and ## p < 0.01 vs. control (0 μM jegosaponin B). (d) Increase of cell membrane permeability of 48 hpf zebrafish by jegosaponin A and B. Purple indicates membrane impermeable DRAQ7-stained nucleus.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.