FIGURE SUMMARY
Title

Spatiotemporal imaging and pharmacokinetics of fluorescent compounds in zebrafish eleuthero-embryos after different routes of administration

Authors
Guarin, M., Faelens, R., Giusti, A., De Croze, N., Léonard, M., Cabooter, D., Annaert, P., de Witte, P., Ny, A.
Source
Full text @ Sci. Rep.
Figure 1

Representative pictures of the spatiotemporal distribution of the fluorescent compound S-CY3A. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 2

Representative pictures of the spatiotemporal distribution of the fluorescent compound S-CY5.5A. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 3

Representative pictures of the spatiotemporal distribution of the fluorescent compound S-CY5A. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 4

Representative pictures of the spatiotemporal distribution of the fluorescent compound FAMA. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 5

Representative pictures of the spatiotemporal distribution of the fluorescent compound TAMRA. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 6

Representative pictures of the spatiotemporal distribution of the fluorescent compound R6GA. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 7

Representative pictures of the spatiotemporal distribution of the fluorescent compound CY3A. The eleuthero-embryos were exposed to the dye by immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and in the yolk sac (IY), or by a combination of immersion and PC microinjection, for 48 h starting from 3 dpf. Staining of neuromast cells of the lateral line is visible 1 h post-immersion. Selected images arranged in MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 8

Schematic illustration of the 1-compartment model used to calculate PK parameters of the fluorescent compounds in the zebrafish eleuthero-embryo. One-way active clearance CL (L/h), passive exchange Q (L/h), compound in the medium (mg/L) and dose administrated by microinjection (mg/kg). The image was drawn using MS PowerPoint and GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 9

Fluorescence-time curves for all compounds and administration routes, with excluded data points marked as X symbols. Model prediction is presented as dotted line, model-predicted equilibrium fluorescence after immersion as solid horizontal line. The image was produced using R (version 4.0.3 https://www.r-project.org/ 2020) and colours arranged in GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.

Figure 10

Relationship between observed RE and LogD for the short incubation (0–3 h) and prolonged incubation period (0–48 h) (a). (b) Plot of the observed RE10/2 versus the predicted RE10/2 in case of the short incubation (0–3 h), (c) and in case of the prolonged incubation period (0–48 h). Dashed line is the line of fit, shade color is the confidence interval of the model (95%). The image was produced using JMP (version 15.1. SAS Institute Inc., Cary, NC, 2019) and arranged in GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Figure 11

Histograms of Relative AUC contribution (RCh) of the immersion and PC exposure route as compared to AUC obtained after combination treatment for a 3 h treatment (a), 24 h treatment (b) and a 48 h treatment (b). The image was produced using JMP (version 15.1. SAS Institute Inc., Cary, NC, 2019) and arranged in GIMP (version 2.10.24 https://www.gimp.org/ 2021) Software.
Acknowledgments
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