FIGURE SUMMARY
Title

Transient activation of the Notch-her15.1 axis plays an important role in the maturation of V2b interneurons

Authors
Mizoguchi, T., Fukada, M., Iihama, M., Song, X., Fukagawa, S., Kuwabara, S., Omaru, S., Higashijima, S.I., Itoh, M.
Source
Full text @ Development
Fig. 1

deltaAdmc72a-mediated changes in Notch signaling affected V2a and V2b cell differentiation. (A-C) Lateral view of vsx2 and gata3 expression in each line. (D,F,H) The immunostaining against Tal1 or Vsx2 in the neural tube in each line. The white arrowheads indicate V2b or V2a cells. (E,G,I) Data analysis of D,F,H.

Fig. 2

Notch activation affects GABAergic neuron differentiation. (A) Lateral view of the immunostaining for GABA in the neural tube of the trunk region of Tg(tal1:EGFP); deltaAdmc72a; Tg(UAS:NICD) embryos at 24 hpf. The white arrowheads indicate V2b cells. The white arrows indicate GABA-negative V2b cells. (B,C) Data analysis of A.

Fig. 3

Forced Notch activation affected V2b neuronal outgrowth. (A) Lateral view of V2b axons in the deltaAdmc72a; Tg(tal1:EGFP) trunk region at 24 hpf. The white dotted lines indicate V2b cell shapes. Arrowheads indicate V2b cell bodies. (B) Data analysis of A.

Fig. 4

her15.1 was expressed in V2b interneurons under Notch signaling. (A) Statistical analysis of her 15.1 expression patterns in V2 neuron pairs. The expression patterns correspond to the expression criteria detailed in Fig. S4A. (B) Representative her15.1 expression patterns in V2 neuron pairs. (C) Expression of her15.1 and tal1 in V2 neuron pairs at 19 ss. (D) her15.1WT, but not her15.1ΔWRPW, exhibited induced tal1 expression in both V2 neuron pairs. (E) her15.1WT overexpression affected axon outgrowth. The magenta dotted lines show the shape of V2 linage interneurons. (F) Data analysis of E. (G) Hypothetical model of V2a and V2b interneuron differentiation and maturation. Notch signaling is transiently activated in the V2b progenitor and induces her15.1. her15.1 might activate downstream genes and direct to V2b fate. After V2b fate determination, Notch signaling is downregulated and the V2b cell matures.

Acknowledgments
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