- Title
-
Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway
- Authors
- Fan, H., Wu, Q., Peng, L., Li, D., Dong, Y., Cao, M., Liu, P., Wang, X., Hu, X., Wang, Y.
- Source
- Full text @ Front Pharmacol
High Performance Liquid Chromatography of PCFF. |
Survival rate of zebrafish. Each value indicates the mean ± SD from three independent experiments. Compared with normal group, *P < 0.05, **P < 0.01. |
Inhibitory effect of PCFF on migration of zebrafish macrophages and neutrophils. Each value indicates the mean ± SD from three independent experiments. Compared with normal group, ##P < 0.01; compared with control group, **P < 0.01. NC group untreated; Model group cut tail; PCFF group added different dose PCFF after tail cut. |
Inhibitory effect of PCFF on expression of iNOS、TNF-α and IL-1β mRNA. Each value indicates the mean ± SD from three independent experiments. Compared with normal group, ##P < 0.01; compared with control group, *P < 0.05, **P < 0.01. NC group untreated; Model group cut tail; PCFF group added different dose PCFF after cut tail. |
Cytotoxicity of PCFF on RAW264.7 cells and effects of PCFF on LPS-induced NO production in RAW264.7 cells. |
Effect of PCFF on LPS-induced inflammatory factor iNOS gene in RAW264.7 cells. Each value indicates the mean ± SD from three independent experiments. Compared with NC group, ##P <0.01; compared with LPS group, **P <0.01. |
Effects of PCFF on TNF-alpha secretion in LPS-induced RAW264.7 cells. Each value indicates the mean ± SD from three independent experiments. Compared with NC group, ##P < 0.01. |
Effects of PCFF on NF-κB and MAPK signaling pathway proteins in LPS-stimulated RAW264.7 macrophages. |