FIGURE SUMMARY
Title

Acute Restraint Stress Evokes Anxiety-Like Behavior Mediated by Telencephalic Inactivation and GabAergic Dysfunction in Zebrafish Brains

Authors
Assad, N., Luz, W.L., Santos-Silva, M., Carvalho, T., Moraes, S., Picanço-Diniz, D.L.W., Bahia, C.P., Oliveira Batista, E.J., da Conceição Passos, A., Oliveira, K.R.H.M., Herculano, A.M.
Source
Full text @ Sci. Rep.
Figure 1

Cell activation in zebrafish brain submitted to ARS. (a) Imunofluorescence of dorsal telencephalon region of zebrafish brain. Top images shows DAPI staining (blue) and c-Fos staining (green) of control group and bottom images represents ARS group. Dm: dorsomedial telencephalon; Dl: dorsolateral telencephalon. Objective: 20x. (b) Counting of the cells marked with c-Fos in the telencephalic region of zebrafish brain. Values showed as MEAN ± S.E.M. (Student’s T test. *p < 0,05).

EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult
Figure 2

Extracellular levels of GABA (a) and glutamate (b) in the brain of zebrafish submitted to ARS. Values showed as MEAN ± S.E.M. (Student’s T test. *p < 0,05).

PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult
Figure 3

Effects of GABA, bicuculline and saclofen in locomotion (a) and behavior parameters of zebrafish: time spent on top in seconds (b) and latency to top in seconds. (c) Animals that did not went through the ARS are represented as S- and those who were submitted to ARS are represented as S+. Values showed as MEAN ± S.E.M. (ANOVA one way, Tukey post test. *Compared to control (S−) #compared to control (S+) p < 0,05).

PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult
Figure 4

Effects of co-treatment of GABA, bicuculline and saclofen in behavior parameters of zebrafish: time spent on top in seconds (a) and latency to top in seconds (b). Animals that did not went through the ARS are represented as S- and those who were submitted to ARS are represented as S+. Values showed as MEAN ± S.E.M. (ANOVA one way, Tukey post test. *Compared to control (S−) #compared to control (S+) p < 0,05).
Figure 5

Representative image of GABAergic signaling in zebrafish brain under normal and ARS conditions.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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