- Title
-
Nanoemulsified adlay bran oil reduces tyrosinase activity and melanin synthesis in B16F10 cells and zebrafish
- Authors
- Ting, Y., Hu, Y.T., Hu, J.Y., Chang, W.C., Huang, Q., Hsieh, S.C.
- Source
- Full text @ Food Sci Nutr
Viability of B16F10 cells after incubation with different surfactants at various concentrations for 48 hr |
(A) Viability and (B) intracellular melanin concentration of B16F10 cells after incubation with adlay bran oil emulsions (at 50 µg/ml) of various particle sizes (100–800 nm) for 48 hr. Different lower case letter indicate significant difference p < 0.05 |
(A) Viability, (B) intracellular melanin concentration, and (C) tyrosinase activity of B16F10 cells after 48 hr of incubation with different concentrations of adlay bran oil emulsion. Different lower case letter indicate significant difference p < 0.05 |
The 48‐hr survival rate of zebrafish cultured with different concentrations of Tween 80. Different lower case letter indicate significant difference p < 0.05 |
(A) Melanin production in zebrafish after culture with adlay bran oil (ABO) emulsions (at 500 µg/ml) of various particle sizes for 48 hr. (B) Photographs of zebrafish cultured in medium containing (a) nothing added (negative control), (b) 0.2 mM 1‐phenyl‐2‐thiourea (positive control), (c) 200 µg/ml Tween 80, and (d–f) 500 µg/ml ABO emulsion with particle sizes of (d) 97.03 nm, (e) 243.8 nm, and (f) 665.5 nm. Different lower case letter indicate significant difference p < 0.05 |
(A) Melanin production and (B) tyrosinase activity in zebrafish after culturing with adlay bran oil (ABO) emulsions of various concentrations for 48 hr. (C) Photographs of zebrafish cultured in medium with (a) nothing added (control), (b) 0.2 mM 1‐phenyl‐2‐thiourea (positive control), (c) 200 µg/ml Tween 80, and (d–f) ABO emulsions at (d) 500 µg/ml, (e) 1,000 µg/ml, and (f) 2,000 µg/ml. Different lower case letter indicate significant difference p < 0.05 |