FIGURE SUMMARY
Title

Effect of microalgae on intestinal inflammation triggered by soybean meal and bacterial infection in zebrafish

Authors
Bravo-Tello, K., Ehrenfeld, N., Solís, C.J., Ulloa, P.E., Hedrera, M., Pizarro-Guajardo, M., Paredes-Sabja, D., Feijóo, C.G.
Source
Full text @ PLoS One
Fig. 1

Effect of microalgae on intestinal inflammation.

(A-G) Lateral view of 9 dpf Tg(BACmpo:GFP)i114 larvae after four days of feeding with the different diets; fishmeal (100FM), soybean meal (50SBM), and soybean meal + microalgae: 50SBM+Ch, 50SBM+Ts, 50SBM+A3Ng, 50SBM+Pt, or 50SBM+No. Black arrowheads indicate neutrophils. (H) The amount of intestinal neutrophils was quantified by immunohistochemistry against GFP. At least 25 larvae per diet were analyzed in each three different experiments. Statistical analysis was conducted using a non-parametric one-way ANOVA. ***P < 0.0001. Red bars represent the mean, and gray bars represent standard deviation.
Fig. 3

Microalgae improve neutrophil behavior after infection.

(A) Assay strategy. (B) Accumulated mortality in challenged (continuous line) and unchallenged (doted line) Tg(BACmpo:GFP)i114 larvae fed with 100FM or 50SBM with and without Tetraselmis sp. (Ts). Mortality was monitored every 12 h until 60 hours post challenge (hpc). (C) Amount of intestinal neutrophils before challenge (T0), and at 60 hpc (T60 Challenge). At least 25 larvae per condition were analyzed in each three independent experiments. Statistical analysis was conducted using a non-parametric Mann-Whitney test. *P < 0.5, ***P < 0.001. Red bars represent the mean, and gray bars represent standard deviation.
Fig. s1

Effect of microalgae on immune response.

(A-G) Lateral view of 9 dpf Tg(BACmpo:GFP)i114 larvae after four days of feeding with the different diets; fishmeal (100FM), soybean meal (50SBM), and fishmeal + microalgae: 100FM+Ch, 100FM+Ts, 100FM+A3Ng, 100FM+Pt, or 100FM+No. Black arrowheads indicate neutrophils. (H) The amount of intestinal neutrophils was quantified by immunohistochemistry against GFP. At least 25 larvae per diet were analyzed in three different experiments. Statistical analysis was conducted using a non-parametric one-way ANOVA. ****P < 0.0001. Red bars represent the mean, and gray bars represent standard deviation.
Fig. s2

Intestinal localization of Edwardsiella tarda after challenge assay.

(A, D). Lateral view of a mid-intestine section from a 12dpf larva. (B, E) mCherry labeled Edwardsiella tarda. (C, F) Merge of both images showing the presence or not of Edwardsiella tarda in the intestine.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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