FIGURE SUMMARY
Title

Shared Segment Analysis and Next-Generation Sequencing Implicates the Retinoic Acid Signaling Pathway in Total Anomalous Pulmonary Venous Return (TAPVR)

Authors
Nash, D., Arrington, C.B., Kennedy, B.J., Yandell, M., Wu, W., Zhang, W., Ware, S., Jorde, L.B., Gruber, P.J., Yost, H.J., Bowles, N.E., Bleyl, S.B.
Source
Full text @ PLoS One
Fig. 3

rbp7a expression in the developing Zebrafish.

rbp7a is strongly expressed within the dorsal forerunner cells (DFCs) and Kupffer’s vesicle (KV) in the developing zebrafish embryo: Panel A. 75% epiboly; Panel B. tailbud stage; Panel C. 8 somite stage.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25
Fig. 4

Effects of rbp7a knockdown on zebrafish heart looping.

Morpholino knockdown of rbp7a results in randomized heart looping. cmlc2 expression at 38 hpf is used to visualize heart looping. Panel A: normal, left-sided heart looping; Panel B: reversed, right-sided heart looping; Panel C: Reversed heart looping occurs in 2% of uninjected control (WT) embryos (n = 315) and 42% of rbp7a morphant (MO) embryos (n = 312).

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25
Fig. 5

Morpholino knockdown of rbp7a results in perturbed spaw expression.

Panel A: representative images showing, left-sided spaw expression, right sided spaw expression, bilateral spaw expression and no spaw expression. Panel B. spaw scoring in WT (blue bars) and rbp7a morphant (MO) embryos (purple bars).

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to 20-25 somites
Fig. 6

KV cilia are shortened in rbp7a morphants.

Panels A and B: Immunohistochemical staining of representative WT and morphant KV and cilia. Panel C: rbp7a morphants have shorter but not fewer KV cilia than WT embryos. * p<0.05.

EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 5-9 somites
Fig. 7

rbp7a and spaw function together in left-right development.

Panel A: Injection of rbp7a (blue bars) or spaw (purple bars) morpholinos at standard concentrations results in significantly altered spaw expression. Panel B: Injection of either morpholino at subthreshold doses (rbp7a: blue bars; spaw: purple bars) results in more normal left-sided spaw expression. However, co-injection of both morpholinos at subthreshold doses leads to significantly altered spaw expression, similar to the higher dose spaw morphants.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: 14-19 somites to 20-25 somites
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.