- Title
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Regulatory back-up circuit of medaka Wt1 co-orthologs ensures PGC maintenance
- Authors
- Klüver, N., Herpin, A., Braasch, I., Driessle, J., and Schartl, M.
- Source
- Full text @ Dev. Biol.
Expression pattern analysis of wt1 genes. (A) Temporal expression pattern of wt1a and wt1b during early medaka development. Zygotic expression starts at stage 11 (Aizawa et al., 2003). (B–M) Spatiotemporal expression pattern of wt1 genes during embryogenesis. The arrowheads mark positive signals of wt1 gene expression domain. g, gut; nd, nephric duct. (N) Expression of wt1 genes in adult tissues. |
Effects of wt1 morpholino knockdown on somatic gonadal mesoderm and PGCs. (A–F) Gene marker expression analysis at stage 33. (G–L) Knockdown of wt1a induces wt1b expression in the wt1a expression domains. The arrowheads mark positive signals of wt1b gene expression in somatic cells of the gonad primordium. g, gut; nd, nephric duct. (M) Real time PCR of wt1b expression in wildtype and wt1a morphants. (N, O) Double knockdown of wt1a and wt1b causes a reduction in PGC numbers. (N, O) Stage 33. |
PGC specification and migration of wildtype and wt1a/b morphant embryos. (A–D) Wild type embryo injected with GFP-3′UTRzfnos mRNA from stages 16–33. (E–H) MOwt1a/b + GFP-32UTRzfnos mRNA injected embryo from stages 16–33. PGCs are specified in wt1a/b morphants and migrate towards the prospective gonadal region. |
Reprinted from Developmental Biology, 325(1), Klüver, N., Herpin, A., Braasch, I., Driessle, J., and Schartl, M., Regulatory back-up circuit of medaka Wt1 co-orthologs ensures PGC maintenance, 179-188, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.