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Fig. 3
Effects of wt1 morpholino knockdown on somatic gonadal mesoderm and PGCs. (A–F) Gene marker expression analysis at stage 33. (G–L) Knockdown of wt1a induces wt1b expression in the wt1a expression domains. The arrowheads mark positive signals of wt1b gene expression in somatic cells of the gonad primordium. g, gut; nd, nephric duct. (M) Real time PCR of wt1b expression in wildtype and wt1a morphants. (N, O) Double knockdown of wt1a and wt1b causes a reduction in PGC numbers. (N, O) Stage 33.
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Reprinted from Developmental Biology, 325(1), Klüver, N., Herpin, A., Braasch, I., Driessle, J., and Schartl, M., Regulatory back-up circuit of medaka Wt1 co-orthologs ensures PGC maintenance, 179-188, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.