Diagram showing the caudal region of embryo to be analyzed (A) and the result image of subtraction between 2 consecutive video frames (B) with yellow pixels representing the dynamic pixels. Schematic diagram illustrated the parameters being analyzed in the signal of dynamic pixels (C). amax: maximum value of dynamic pixels; amin: minimum value of dyannmic pixels; heart beat interval: the time for a complete heart beat between 2 toughs; Scale Bar: 50 μm; Right to anterior (head).

Acknowledgments
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