ZFIN Lab: Tamplin Lab

ZFIN ID: ZDB-LAB-160815-1

ZFIN ID: ZDB-LAB-160815-1

Tamplin Lab

PI/Director:	Tamplin, Owen
Contact Person:	Tamplin, Owen
Email:	tamplin@wisc.edu
URL:	https://tamplin.crb.wisc.edu/
Address:	Department of Cell & Regenerative Biology School of Medicine and Public Health University of Wisconsin-Madison 1111 Highland Ave 4459 WIMR II Madison, WI 53705
Country:	United States
Phone:	312-355-1138
Fax:
Line Designation:	None assigned

GENOMIC FEATURES ORIGINATING FROM THIS LAB No data available

STATEMENT OF RESEARCH INTERESTS

In the Tamplin lab, we study the basic biology of hematopoietic stem cells (HSC) and their microenvironment. These stem cells are clinically important as a curative treatment for many blood cancers and diseases. A better understanding of HSC and their supportive niche will help us improve these treatments and thereby patient survival.

We use complementary mouse and zebrafish model organisms. The zebrafish is now a well-established model for in vivo genetic and chemical screening. It is the only vertebrate genetic model that allows high-resolution live imaging of endogenous cellular behaviors. We can harness the strengths of the zebrafish model to develop and test unique hypotheses that could not be acquired using any other system. We then use the mouse model to test translation of our findings to a mammalian system.

Our experimental approaches integrate genetic tools, live imaging, and chemical biology. We use highly specific transgenic fluorescent reporters of HSC to directly observe endogenous stem cells in their niche. Together with reporters of other cell types, such as endothelial and stromal cells, we can track single stem cells as they interact with their surrounding cells. The goal of this research is to understand the mechanisms of communication between HSC and the niche.

LAB MEMBERS

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Agarwala, S., Kim, K.Y., Phan, S., Ju, S., Kong, Y.E., Castillon, G.A., Bushong, E.A., Ellisman, M.H., Tamplin, O.J. (2022) Defining the ultrastructure of the hematopoietic stem cell niche by correlative light and electron microscopy. eLIFE. 11:

Schiavo, R.K., Tamplin, O.J. (2021) Vascular endothelial growth factor-c regulates hematopoietic stem cell fate in the dorsal aorta. Development (Cambridge, England). 149(2):

Tamplin, O.J. (2020) Making fish a little more human: a zebrafish hematopoietic xenotransplant model is improved by the expression of human cytokines. Haematologica. 105:2346-2347

Li, P., Lahvic, J.L., Binder, V., Pugach, E.K., Riley, E.B., Tamplin, O.J., Panigrahy, D., Bowman, T.V., Barrett, F.G., Heffner, G.C., McKinney-Freeman, S., Schlaeger, T.M., Daley, G.Q., Zeldin, D.C., Zon, L.I. (2019) Author Correction: Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment. Nature. 573:E1

Blaser, B.W., Moore, J.L., Hagedorn, E.J., Li, B., Riquelme, R., Lichtig, A., Yang, S., Zhou, Y., Tamplin, O.J., Binder, V., Zon, L.I. (2017) CXCR1 remodels the vascular niche to promote hematopoietic stem and progenitor cell engraftment. The Journal of experimental medicine. 214(4):1011

Anderson, H., Patch, T.C., Reddy, P.N., Hagedorn, E.J., Kim, P.G., Soltis, K.A., Chen, M.J., Tamplin, O.J., Frye, M., MacLean, G.A., Hübner, K., Bauer, D.E., Kanki, J.P., Vogin, G., Huston, N.C., Nguyen, M., Fujiwara, Y., Paw, B.H., Vestweber, D., Zon, L.I., Orkin, S.H., Daley, G.Q., Shah, D.I. (2015) Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression. Blood. 126(26):2811-20

Li, P., Lahvic, J.L., Binder, V., Pugach, E.K., Riley, E.B., Tamplin, O.J., Panigrahy, D., Bowman, T.V., Barrett, F.G., Heffner, G.C., McKinney-Freeman, S., Schlaeger, T.M., Daley, G.Q., Zeldin, D.C., Zon, L.I. (2015) Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment. Nature. 523:468-71

Jing, L., Tamplin, O.J., Chen, M.J., Deng, Q., Patterson, S., Kim, P.G., Durand, E.M., McNeil, A., Green, J.M., Matsuura, S., Ablain, J., Brandt, M.K., Schlaeger, T.M., Huttenlocher, A., Daley, G.Q., Ravid, K., Zon, L.I. (2015) Adenosine signaling promotes hematopoietic stem and progenitor cell emergence. The Journal of experimental medicine. 212(5):649-63

Tamplin, O.J., Durand, E.M., Carr, L.A., Childs, S.J., Hagedorn, E.J., Li, P., Yzaguirre, A.D., Speck, N.A., Zon, L.I. (2015) Hematopoietic Stem Cell Arrival Triggers Dynamic Remodeling of the Perivascular Niche. Cell. 160:241-252

Cooney, J.D., Hildick-Smith, G.J., Shafizadeh, E., McBride, P.F., Carroll, K.J., Anderson, H., Shaw, G.C., Tamplin, O.J., Branco, D.S., Dalton, A.J., Shah, D.I., Wong, C., Gallagher, P.G., Zon, L.I., North, T.E., and Paw, B.H. (2013) Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis. Developmental Biology. 373(2):431-441

Tamplin, O.J., White, R.M., Jing, L., Kaufman, C.K., Lacadie, S.A., Li, P., Taylor, A.M., and Zon, L.I. (2012) Small molecule screening in zebrafish: swimming in potential drug therapies. Wiley Interdisciplinary Reviews: Developmental Biology. 1(3):459-468

Tamplin, O.J., Cox, B.J., and Rossant, J. (2011) Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Developmental Biology. 360(2):415-25

Mosimann, C., Kaufman, C.K., Li, P., Pugach, E.K., Tamplin, O.J., and Zon, L.I. (2011) Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish. Development (Cambridge, England). 138(1):169-177

Tamplin, O.J., and Zon, L.I. (2010) Blood flow: metalloproteases cut loose in primitive erythrocytes. Current biology : CB. 20(13):R561-R562