ZFIN Lab: Department of Organismal Animal Physiology

ZFIN ID: ZDB-LAB-101208-1

Department of Organismal Animal Physiology

PI/Director:	Flik, Gert
Co-PI / Senior Researcher:	Klaren, Peter
Contact Person:	Maurits, Daisy
Email:	d.maurits@science.ru.nl
URL:	http://www.organphy.science.ru.nl/
Address:	Department of Organismal Animal Physiology Institute for Water and Wetland Research, Faculty of Science, Radboud University Nijmegen
Country:	Netherlands
Phone:	+31 24 3653244
Fax:	+31 24 3653229
Line Designation:	rdb

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 2 genomic features

STATEMENT OF RESEARCH INTERESTS

Stress physiology, thyroidology, energie metabolism, bone metabolism, aquaculture and welfare.

LAB MEMBERS

Arjona, F.J. Post-Doc	Metz, Juriaan R. Post-Doc	Boerrigter, Jeroen Graduate Student
Gorissen, Marnix Graduate Student	Manuel, Ing. Remy Graduate Student	Roques, Jonathan Graduate Student
van Kessel, Maartje Graduate Student	Spanings, Tom Fish Facility Staff	Atsma, Wim Technical Staff
Maurits, Daisy Administrative Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Mes, W., Lücker, S., Jetten, M.S.M., Siepel, H., Gorissen, M., van Kessel, M.A.H.J. (2023) Comparison of the gill and gut microbiomes of common carp (Cyprinus carpio) and zebrafish (Danio rerio) and their RAS environment. The Science of the total environment. 896:165212

Raterman, S.T., Von Den Hoff, J.W., Dijkstra, S., De Vriend, C., Te Morsche, T., Broekman, S., Zethof, J., De Vrieze, E., Wagener, F.A.D.T.G., Metz, J.R. (2023) Disruption of the foxe1 gene in zebrafish reveals conserved functions in development of the craniofacial skeleton and the thyroid. Frontiers in cell and developmental biology. 11:11438441143844

Gebuijs, L., Wagener, F.A., Zethof, J., Carels, C.E., Von den Hoff, J.W., Metz, J.R. (2022) Targeting fibroblast growth factor receptors causes severe craniofacial malformations in zebrafish larvae. PeerJ. 10:e14338e14338

Bergen, D.J.M., Tong, Q., Shukla, A., Newham, E., Zethof, J., Lundberg, M., Ryan, R., Youlten, S.E., Frysz, M., Croucher, P.I., Flik, G., Richardson, R.J., Kemp, J.P., Hammond, C.L., Metz, J.R. (2022) Regenerating zebrafish scales express a subset of evolutionary conserved genes involved in human skeletal disease. BMC Biology. 20:21

Dona, M., Lamers, M., Rohde, S., Gorissen, M., Timmers, H.J.L.M. (2021) Targeting the Redox Balance Pathway Using Ascorbic Acid in sdhb Zebrafish Mutant Larvae. Cancers. 13(20):

Bek, J.W., Shochat, C., De Clercq, A., De Saffel, H., Boel, A., Metz, J., Rodenburg, F., Karasik, D., Willaert, A., Coucke, P.J. (2021) Lrp5 mutant and crispant zebrafish faithfully model human osteoporosis, establishing the zebrafish as a platform for CRISPR-based functional screening of osteoporosis candidate genes. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 36(9):1749-1764

Raterman, S.T., Metz, J.R., Wagener, F.A.D.T.G., Von den Hoff, J.W. (2020) Zebrafish Models of Craniofacial Malformations: Interactions of Environmental Factors. Frontiers in cell and developmental biology. 8:600926

Dona, M., Waaijers, S., Richter, S., Eisenhofer, G., Korving, J., Kamel, S.M., Bakkers, J., Rapizzi, E., Rodenburg, R.J., Zethof, J., Gorissen, M., Flik, G., Deen, P.M.T., Timmers, H.J. (2020) Loss of sdhb in zebrafish larvae recapitulates human paraganglioma characteristics. Endocrine-related cancer. 28(1):65-77

Zhang, H., Waldmann, L., Manuel, R., Boije, H., Haitina, T., Allalou, A. (2020) zOPT: an open source optical projection tomography system and methods for rapid 3D zebrafish imaging. Biomedical Optics Express. 11:4290-4305

Gebuijs, I.G.E., Metz, J.R., Zethof, J., Carels, C.E.L., Wagener, F.A.D.T.G., Von den Hoff, J.W. (2020) The anti-epileptic drug valproic acid causes malformations in the developing craniofacial skeleton of zebrafish larvae. Mechanisms of Development. 163:103632

Del Pozo Cano, A., Manuel, R., Iglesias Gonzalez, A.B., Koning, H., Habicher, J., Zhang, H., Allalou, A., Kullander, K., Boije, H. (2020) Behavioural characterization of dmrt3a mutant zebrafish reveals crucial aspects of vertebrate locomotion through phenotypes related to acceleration. eNeuro. 7(3):

van den Bos, R., Cromwijk, S., Tschigg, K., Althuizen, J., Zethof, J., Whelan, R., Flik, G., Schaaf, M. (2020) Early Life Glucocorticoid Exposure Modulates Immune Function in Zebrafish (Danio rerio) Larvae. Frontiers in immunology. 11:727

Verschuren, E.H.J., Rigalli, J.P., Castenmiller, C., Rohrbach, M.U., Bindels, R.J.M., Peters, D.J.M., Arjona, F.J., Hoenderop, J.G.J. (2020) Pannexin-1 mediates fluid shear stress-sensitive purinergic signaling and cyst growth in polycystic kidney disease. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 34(5):6382-6398

van de Pol, I.L.E., Flik, G., Verberk, W.C.E.P. (2020) Triploidy in zebrafish larvae: Effects on gene expression, cell size and cell number, growth, development and swimming performance. PLoS One. 15:e0229468

Gebuijs, I.G.E., Raterman, S.T., Metz, J.R., Swanenberg, L., Zethof, J., Van den Bos, R., Carels, C.E.L., Wagener, F.A.D.T.G., Von den Hoff, J.W. (2019) Fgf8a mutation affects craniofacial development and skeletal gene expression in zebrafish larvae. Biology Open. 8(9):

Groeneweg, S., Kersseboom, S., van den Berge, A., Dolcetta-Capuzzo, A., van Geest, F.S., van Heerebeek, R.E.A., Arjona, F.J., Meima, M., Peeters, R., Visser, W.E., Visser, T.J. (2019) In vitro characterization of human, mouse and zebrafish MCT8 orthologues. Thyroid : official journal of the American Thyroid Association. 29(10):1499-1510

van den Bos, R., Althuizen, J., Tschigg, K., Bomert, M., Zethof, J., Filk, G., Gorissen, M. (2019) Early life exposure to cortisol in zebrafish (Danio rerio): similarities and differences in behaviour and physiology between larvae of the AB and TL strains. Behavioural pharmacology. 30(2 and 3 - Special Issue):260-271

San, B., Rougeot, J., Voeltzke, K., van Vegchel, G., Aben, M., Andralojc, K.M., Flik, G., Kamminga, L.M. (2019) The ezh2(sa1199) mutant zebrafish display no distinct phenotype. PLoS One. 14:e0210217

Arjona, F.J., Latta, F., Mohammed, S.G., Thomassen, M., van Wijk, E., Bindels, R.J.M., Hoenderop, J.G.J., de Baaij, J.H.F. (2018) SLC41A1 is essential for magnesium homeostasis in vivo. Pflugers Archiv : European journal of physiology. 471(6):845-860

Corre, T., Arjona, F.J., Hayward, C., Youhanna, S., de Baaij, J.H.F., Belge, H., Nägele, N., Debaix, H., Blanchard, M.G., Traglia, M., Harris, S.E., Ulivi, S., Rueedi, R., Lamparter, D., Macé, A., Sala, C., Lenarduzzi, S., Ponte, B., Pruijm, M., Ackermann, D., Ehret, G., Baptista, D., Polasek, O., Rudan, I., Hurd, T.W., Hastie, N.D., Vitart, V., Waeber, G., Kutalik, Z., Bergmann, S., Vargas-Poussou, R., Konrad, M., Gasparini, P., Deary, I.J., Starr, J.M., Toniolo, D., Vollenweider, P., Hoenderop, J.G.J., Bindels, R.J.M., Bochud, M., Devuyst, O. (2017) Genome-Wide Meta-Analysis Unravels Interactions between Magnesium Homeostasis and Metabolic Phenotypes. Journal of the American Society of Nephrology : JASN. 29(1):335-348

van den Bos, R., Zethof, J., Flik, G., Gorissen, M. (2017) Light regimes differentially affect baseline transcript abundance of stress-axis and (neuro)development-related genes in zebrafish (Danio rerio, Hamilton 1822) AB and TL larvae.. Biology Open. 6(11):1692-1697

van den Bos, R., Mes, W., Galligani, P., Heil, A., Zethof, J., Flik, G., Gorissen, M. (2017) Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS One. 12:e0175420

Kersten, S., Arjona, F.J. (2017) Ion transport in the zebrafish kidney from a human disease angle: possibilities, considerations and future perspectives. American journal of physiology. Renal physiology. 312(1):F172-F189

Manuel, R., Zethof, J., Flik, G., Bos, R.V. (2015) Providing a food reward reduces inhibitory avoidance learning in zebrafish. Behavioural processes. 120:69-72

Sharif, F., Steenbergen, P.J., Metz, J.R., Champagne, D.L. (2015) Long-lasting effects of dexamethasone on immune cells and wound healing in the zebrafish. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 23(6):855-65

Dona, M., Bachmann-Gagescu, R., Texier, Y., Toedt, G., Hetterschijt, L., Tonnaer, E.L., Peters, T.A., van Beersum, S.E., Bergboer, J.G., Horn, N., de Vrieze, E., Slijkerman, R.W., van Reeuwijk, J., Flik, G., Keunen, J.E., Ueffing, M., Gibson, T.J., Roepman, R., Boldt, K., Kremer, H., van Wijk, E. (2015) NINL and DZANK1 Co-function in Vesicle Transport and Are Essential for Photoreceptor Development in Zebrafish. PLoS Genetics. 11:e1005574

Gorissen, M., Manuel, R., Pelgrim, T.N., Mes, W., de Wolf, M.J., Zethof, J., Flik, G., van den Bos, R. (2015) Differences in inhibitory avoidance, cortisol and brain gene expression in TL and AB zebrafish. Genes, brain, and behavior. 14(5):428-38

de Vrieze, E., Zethof, J., Schulte-Merker, S., Flik, G., Metz, J.R. (2015) Identification of novel osteogenic compounds by an ex-vivo Sp7:luciferase zebrafish scale assay. Bone. 74:106-13

Manuel, R., Gorissen, M., Stokkermans, M., Zethof, J., Ebbesson, L.O., Vis, H.V., Flik, G., Bos, R.V. (2015) The Effects of Environmental Enrichment and Age-Related Differences on Inhibitory Avoidance in Zebrafish (Danio rerio Hamilton). Zebrafish. 12(2):152-65

Manuel, R., Gorissen, M., Zethof, J., Ebbesson, L.O., van de Vis, H., Flik, G., van den Bos, R. (2014) Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase. The Journal of experimental biology. 217(Pt 21):3919-28

Boerrigter, J.G., van de Vis, H.W., van den Bos, R., Abbink, W., Spanings, T., Zethof, J., Martinez, L.L., van Andel, W.F., Lopez-Luna, J., and Flik, G. (2014) Effects of Pro-Tex on zebrafish (Danio rerio) larvae, adult common carp (Cyprinus carpio) and adult yellowtail kingfish (Seriola lalandi). Fish physiology and biochemistry. 40(4):1201-12

Manuel, R., Gorissen, M., Piza Roca, C., Zethof, J., Vis, H.V., Flik, G., Bos, R.V. (2014) Inhibitory Avoidance Learning in Zebrafish (Danio Rerio): Effects of Shock Intensity and Unraveling Differences in Task Performance. Zebrafish. 11(4):341-52

de Vrieze, E., van de Wiel, S.M., Zethof, J., Flik, G., Klaren, P.H., Arjona, F.J. (2014) Knockdown of monocarboxylate transporter 8 (mct8) disturbs brain development and locomotion in zebrafish. Endocrinology. 155(6):2320-30

Arjona, F.J., de Baaij, J.H., Schlingmann, K.P., Lameris, A.L., van Wijk, E., Flik, G., Regele, S., Korenke, G.C., Neophytou, B., Rust, S., Reintjes, N., Konrad, M., Bindels, R.J., Hoenderop, J.G. (2014) CNNM2 Mutations Cause Impaired Brain Development and Seizures in Patients with Hypomagnesemia. PLoS Genetics. 10:e1004267

de Vrieze, E., Moren, M., Metz, J.R., Flik, G., Lie, K.K. (2014) Arachidonic acid enhances turnover of the dermal skeleton: studies on zebrafish scales. PLoS One. 9:e89347

de Vrieze, E., van Kessel, M.A., Peters, H.M., Spanings, F.A., Flik, G., and Metz, J.R. (2014) Prednisolone induces osteoporosis-like phenotype in regenerating zebrafish scales. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA. 25(2):567-78

Arjona, F.J., Chen, Y.X., Flik, G., Bindels, R.J., and Hoenderop, J.G. (2013) Tissue-specific expression and in vivo regulation of zebrafish orthologues of mammalian genes related to symptomatic hypomagnesemia. Pflugers Archiv : European journal of physiology. 465(10):1409-21

Huitema, L.F., Renn, J., Logister, I., Gray, J.K., Waltz, S.E., Flik, G., and Schulte-Merker, S. (2012) Macrophage-stimulating protein and calcium homeostasis in zebrafish. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 26(10):4092-4101

Louwette, S., Labarque, V., Wittevrongel, C., Thys, C., Metz, J., Gijsbers, R., Debyser, Z., Arnout, J., Van Geet, C., and Freson, K. (2012) Regulator of G-protein signaling 18 controls megakaryopoiesis and the cilia-mediated vertebrate mechanosensory system. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 26(5):2125-2136

Wunderink, Y.S., de Vrieze, E., Metz, J.R., Halm, S., Martínez-Rodríguez, G., Flik, G., Klaren, P.H., and Mancera, J.M. (2012) Subfunctionalization of POMC paralogues in Senegalese sole (Solea senegalensis). General and comparative endocrinology. 175(3):407-415

Arjona, F.J., de Vrieze, E., Visser, T.J., Flik, G., and Klaren, P.H. (2011) Identification and Functional Characterization of Zebrafish Solute Carrier Slc16a2 (Mct8) as a Thyroid Hormone Membrane Transporter. Endocrinology. 152(12):5065-73

Vanoevelen, J., Janssens, A., Huitema, L.F., Hammond, C.L., Metz, J.R., Flik, G., Voets, T., and Schulte-Merker, S. (2011) Trpv5/6 is vital for epithelial calcium uptake and bone formation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 25(9):3197-207

de Vrieze, E., Sharif, F., Metz, J.R., Flik, G., and Richardson, M.K. (2011) Matrix metalloproteinases in osteoclasts of ontogenetic and regenerating zebrafish scales. Bone. 48(4):704-712

Gorissen, M., Bernier, N., Nabuurs, S., Flik, G., and Huising, M. (2009) Two divergent leptin paralogues in zebrafish (Danio rerio) that originate early in teleostean evolution. The Journal of endocrinology. 201(3):329-339

Sánchez, E., Rubio, V.C., Thompson, D.A., Metz, J., Flik, G., Millhauser, G.L., and Cerda-Reverter, J.M. (2009) Phosphodiesterase inhibitor-dependent inverse agonism of agouti-related protein on melanocortin 4 receptor in sea bass (Dicentrarchus labrax). American journal of physiology. Regulatory, integrative and comparative physiology. 296(5):R1293-R1306

Brittijn, S.A., Duivesteijn, S.J., Belmamoune, M., Bertens, L.F., Bitter, W., Debruijn, J.D., Champagne, D.L., Cuppen, E., Flik, G., Vandenbroucke-Grauls, C.M., Janssen, R.A., de Jong, I.M., de Kloet, E.R., Kros, A., Meijer, A.H., Metz, J.R., van der Sar, A.M., Schaaf, M.J., Schulte-Merker, S., Spaink, H.P., Tak, P.P., Verbeek, F.J., Vervoordeldonk, M.J., Vonk, F.J., Witte, F., Yuan, H., and Richardson, M.K. (2009) Zebrafish development and regeneration: new tools for biomedical research. The International journal of developmental biology. 53(5-6):835-850

Huising, M.O., Geven, E.J., Kruiswijk, C.P., Nabuurs, S.B., Stolte, E.H., Spanings, F.A., Verburg-van Kemenade, B.M., and Flik, G. (2006) Increased leptin expression in common Carp (Cyprinus carpio) after food intake but not after fasting or feeding to satiation. Endocrinology. 147(12):5786-5797

Huising, M.O., Kruiswijk, C.P., and Flik, G. (2006) Phylogeny and evolution of class-I helical cytokines. The Journal of endocrinology. 189(1):1-25

Huising, M.O., van Schijndel, J.E., Kruiswijk, C.P., Nabuurs, S.B., Savelkoul, H.F., Flik, G., and Verburg-van Kemenade, B.M. (2006) The presence of multiple and differentially regulated interleukin-12p40 genes in bony fishes signifies an expansion of the vertebrate heterodimeric cytokine family. Molecular immunology. 43(10):1519-1533

Huising, M.O., Kruiswijk, C.P., van Schijndel, J.E., Savelkoul, H.F., Flik, G., and Verburg-van Kemenade, B.M. (2005) Multiple and highly divergent IL-11 genes in teleost fish. Immunogenetics. 57(6):432-443

Huising, M.O., Metz, J.R., van Schooten, C., Taverne-Thiele, A.J., Hermsen, T., Verburg-van Kemenade, B.M., and Flik, G. (2004) Structural characterisation of a cyprinid (Cyprinus carpio L.) CRH, CRH-BP and CRH-R1, and the role of these proteins in the acute stress response. Journal of molecular endocrinology. 32(3):627-648