- Title
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Dual-color deep-tissue three-photon microscopy with a multiband infrared laser
- Authors
- Guesmi, K., Abdeladim, L., Tozer, S., Mahou, P., Kumamoto, T., Jurkus, K., Rigaud, P., Loulier, K., Dray, N., Georges, P., Hanna, M., Livet, J., Supatto, W., Beaurepaire, E., Druon, F.
- Source
- Full text @ Light Sci Appl
a In vivo imaging through the skull in adult zebrafish telencephalon. The figures show representative XY, XZ, and 3D views for a volume encompassing two labeled cell populations: red dTomato-labeled radial glia and green GFP-labeled neural stem cells expressing the deltaA neurogenic gene (see Materials and methods section). Simultaneously acquired THG signals provide additional label-free contextual information (skin and skull morphology, blood vessels, lipid accumulations). See also Movies 7 and 8 and related information. b Simultaneous dual-color 3PEF and THG imaging of developing chick embryo spinal cord tissue (stage E3) expressing cytoplasmic GFP labeling and nuclear RFP labeling. The images are extracted from a 2-h-long experiment and illustrate developmental processes such as cell migration and process formation. See also Movies 5 and 6 and related information. Scale bars, 100 µm |