Sarangdhar et al., 2018 - Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment. Nucleic acids research   46(18):9726-9735 Full text @ Nucleic Acids Res.

Fig. 2 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 3

Inheritance of conserved lncRNA Cyrano in zebrafish. (A) FPKM values of Cyrano expression in RNA-seq datasets of zebrafish testis, sperm, oocyte, 2cell-stage shows that Cyrano is present as early as 2cell-stage in zebrafish embryo. (B) RT-PCR validation of Cyrano and unspliced Cyrano expression in zebrafish testis, oocyte and 2cell-stage. (C) qPCR validation of Cyrano and unspliced Cyrano expression in zebrafish developmental stages. (D) Expression of Cyrano in zebrafish developmental stages checked by In situ hybridization. Error bar represents standard error of mean from N = number of independent biological replicates. *P value ≤0.05.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 5

Ectopic over-expression of miR-7 leads to defective ventricle opening and it is rescued by Cyrano. First panel shows control mimic injected zebrafish embryos, second panel with 300 pg of miR-7 mimic injected embryos, third panel has embryos injected with 6 pg of Cyrano transcript and 300 pg of miR-7 mimic and fourth panel has embryos injected with 6 pg of miR-7 resistant Cyrano transcript (miR-7 res Cyrano) along with 300 pg of miR-7 mimic imaged at 24 hpf. Defects were observed (shown by dotted arrow) in 70 out of 124 animals with miR-7 mimic injection and these defects were restricted to 5 out of 23 and 13 out of 48 animals after rescue with wild type Cyrano or miR-7 res Cyrano, respectively. Forebrain (F), Midbrain (M), Hindbrain (H) ventricles shown by dotted lines.

Fig. 6

Loss of inherited Cyrano by ectopic over-expression of miR-7 leads to defective brain development in 24 hpf embryos. (A) HuC: kaede zebrafish injected with control or miR-7 mimic imaged at 24 hpf to capture GFP labelled medial longitudinal fasciculus (MLF), nucleus of medial longitudinal fasciculus (nMLF) and trigeminal ganglion (tg). (B) Distance between MLF and (C) nMLF in control and miR-7 mimic over-expressed embryos measured, both the regions shown by red boxes in A. (D) Distance between left tg to nMLF and (E) right tg to nMLF in control and miR-7 mimic over-expressed embryos measured, as shown by red lines in A. Error bar represents standard error of mean, n = number of animals, **P value ≤ 0.01, *P value ≤ 0.05.

Fig. 7

Effect of loss of inherited Cyrano by ectopic over-expression of miR-7 in 48 hpf embryos. (A) Tg[HuC:kaede X her4.1:mCherry] zebrafish embryos injected with control or miR-7 mimic imaged at 48 hpf to capture optic tectum and Upper Rhombic Lip (URL). (B) Distance between left and right optic tectum and (C) URL in control and miR-7 mimic over-expressed embryos measured, both the regions marked by black dotted lines in A. Error bar represents standard error of mean, n = number of animals, **P value ≤ 0.01, *P value ≤ 0.05.

Acknowledgments:
ZFIN wishes to thank the journal Nucleic acids research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nucleic Acids Res.