PUBLICATION
Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment
- Authors
- Sarangdhar, M.A., Chaubey, D., Srikakulam, N., Pillai, B.
- ID
- ZDB-PUB-180717-9
- Date
- 2018
- Source
- Nucleic acids research 46(18): 9726-9735 (Journal)
- Registered Authors
- Chaubey, Divya, Pillai, Beena, Sarangdhar, Mayuresh A.
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Brain/embryology*
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Gene Regulatory Networks
- High-Throughput Nucleotide Sequencing
- Inheritance Patterns/genetics
- MicroRNAs/physiology
- RNA, Long Noncoding/physiology*
- RNA, Messenger, Stored/genetics
- RNA, Messenger, Stored/metabolism
- Sequence Analysis, RNA
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 30011017 Full text @ Nucleic Acids Res.
Citation
Sarangdhar, M.A., Chaubey, D., Srikakulam, N., Pillai, B. (2018) Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment. Nucleic acids research. 46(18):9726-9735.
Abstract
Transfer of genetic material from parents to progeny via fusion of gametes is a way to ensure flow of information from one generation to the next. Apart from the genetic material, gametes provide a rich source of other factors such as RNA and proteins which can control traits of the embryo. Non-coding RNAs are not only carriers of regulatory information but can also encode memory of events of parental life. Here, we explore the possibility of parental inheritance of non-coding RNAs, especially long non-coding RNAs. Meta-analysis of RNA-seq data revealed several non-coding RNAs present in zebrafish oocyte, sperm and 2cell-stage. The embryo is transcriptionally silent at this stage, we rationalize that all the RNAs detectable at 2cell-stage are deposited either by sperm or oocyte or both and thus inherited. In the inherited pool, we noticed a conserved lncRNA, Cyrano previously known for zebrafish brain development. Knockdown of inherited Cyrano by miR-7 without changing zygotic Cyrano altered brain morphology at 24 hpf and 48 hpf. This defect could be partially rescued by injecting full length Cyrano lncRNA or a mutant resilient to knock-down by miR-7. In future, there is ample scope to check the possibility of inherited lncRNAs as carriers of memory of parental life events and building blocks that set up an initial platform for development.
Errata / Notes
This article is corrected by ZDB-PUB-220819-6.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping