ZFIN ID: ZDB-PUB-180717-9
Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment
Sarangdhar, M.A., Chaubey, D., Srikakulam, N., Pillai, B.
Date: 2018
Source: Nucleic acids research   46(18): 9726-9735 (Journal)
Registered Authors: Chaubey, Divya, Pillai, Beena, Sarangdhar, Mayuresh A.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain/embryology*
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Gene Regulatory Networks
  • High-Throughput Nucleotide Sequencing
  • Inheritance Patterns/genetics
  • MicroRNAs/physiology
  • RNA, Long Noncoding/physiology*
  • RNA, Messenger, Stored/genetics
  • RNA, Messenger, Stored/metabolism
  • Sequence Analysis, RNA
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 30011017 Full text @ Nucleic Acids Res.
Transfer of genetic material from parents to progeny via fusion of gametes is a way to ensure flow of information from one generation to the next. Apart from the genetic material, gametes provide a rich source of other factors such as RNA and proteins which can control traits of the embryo. Non-coding RNAs are not only carriers of regulatory information but can also encode memory of events of parental life. Here, we explore the possibility of parental inheritance of non-coding RNAs, especially long non-coding RNAs. Meta-analysis of RNA-seq data revealed several non-coding RNAs present in zebrafish oocyte, sperm and 2cell-stage. The embryo is transcriptionally silent at this stage, we rationalize that all the RNAs detectable at 2cell-stage are deposited either by sperm or oocyte or both and thus inherited. In the inherited pool, we noticed a conserved lncRNA, Cyrano previously known for zebrafish brain development. Knockdown of inherited Cyrano by miR-7 without changing zygotic Cyrano altered brain morphology at 24 hpf and 48 hpf. This defect could be partially rescued by injecting full length Cyrano lncRNA or a mutant resilient to knock-down by miR-7. In future, there is ample scope to check the possibility of inherited lncRNAs as carriers of memory of parental life events and building blocks that set up an initial platform for development.