- Title
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The Role of Glypicans in Wnt Inhibitory Factor-1 Activity and the Structural Basis of Wif1's Effects on Wnt and Hedgehog Signaling
- Authors
- Avanesov, A., Honeyager, S.M., Malicki, J., and Blair, S.S.
- Source
- Full text @ PLoS Genet.
Gpc4 enhances the effects of full-length Wif1 in zebrafish embryos. Approximately 2 nL of mRNA of a given concentration was injected into one cell stage embryos, and embryos were scored at 28–30 hours post-fertilization. Images show representative examples of the penetrance of the short-tailed phenotype compared to an uninjected control. In top panels dorsal is up and anterior is to the left. Bar graphs show percentage of short-tailed embryos. The data is pooled from two independent experiments; frequencies were scored and their percentages were averaged. See Materials and Methods for information on RNA preparation. |
Wif1 expression in zebrafish embryos leads to decreased expression of a β-catenin-regulated reporter. (A, A′) DIC (A) and fluorescence (A′) images of embryos carrying the Tg(TOP:dGFP) reporter, showing GFP expression in the dorsal midbrain (arrow), anterior to the midbrain-hindbrain boundary. (B) Injection of 40 pg/nL of wif1 mRNA reduces GFP expression (arrow). (C) Injection of 5 pg/nL of kny mRNA does not change GFP expression. (D) GFP intensities were measured in living embryos and their statistically significant differences were verified by two-tailed Mann-Whitney U test. Statistically significant differences in GFP intensity were measured. No differences of statistical significance from were observed between wild-type and kny-injected embryos. |