Transgenic Construct
Tg(E1B:Hsa.ATXN1[82Q],E1B:GAP-mScarlet)
- ID
- ZDB-TGCONSTRCT-220822-3
- Name
- Tg(E1B:Hsa.ATXN1[82Q],E1B:GAP-mScarlet)
- Previous Names
- None
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- Contains Sequences
- None
- Note
-
This construct was designed to express a pathological human patient-derived allele of Ataxin 1 in zebrafish Purkinje cells. The mutant sequence contains 82 non-interrupted glutamine residues ( Atx1[82Q] ), designed to model SCA1. An HA-tag is fused to the N-terminus for immunohistochemical detection of the transgene product
Plasmid Map
None
Genomic Features
That Utilize tg(e1b:hsa.atxn1[82q],e1b:gap-mscarlet) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| bz22Tg |
Transgenics
That Utilize tg(e1b:hsa.atxn1[82q],e1b:gap-mscarlet) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| bz12Tg; bz22Tg/+ |
|
Figure 3
from
Elsaey et al., 2021
|
3 figures
from
Elsaey et al., 2021
|
| bz22Tg/+ |
|
2 figures
from
Elsaey et al., 2021
|
Figure 4
from
Elsaey et al., 2021
|
Sequence Information
Other Construct Pages
None
Citations