Transgenic Construct
Tg(pvalb9:Hsa.CLRN1_c.144T>G-YFP)
- ID
- ZDB-TGCONSTRCT-161017-2
- Name
- Tg(pvalb9:Hsa.CLRN1_c.144T>G-YFP)
- Previous Names
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:EYFP
- Contains Sequences
- Note
-
In this construct, pvalb9 promoter sequence drives expression in zebrafish hair cells of the pathogenic variant c.144T>G in human clarin1 (CLRN1). In humans, this variant causes Usher syndrome IIIA (USH3A), a rare genetic disorder that results in progressive loss of vision and hearing. c.144T>G substitutes lysine (K) for asparagine (N) at position 48, a conserved N-glycosylation site, resulting in glycosylation-deficient CLRN1 which is retained in the endoplasmic reticulum (ER) and is prone to degradation by the proteasome
Plasmid Map
None
Genomic Features
That Utilize Tg(pvalb9:Hsa.CLRN1_c.144T>G-YFP) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| cwr1006Tg |
Transgenics
That Utilize Tg(pvalb9:Hsa.CLRN1_c.144T>G-YFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| clrn1cwr1003/cwr1003; cwr1006Tg | 2 figures from Gopal et al., 2019 | Fig. 2 from Gopal et al., 2019 | |
| cwr1006Tg |
|
2 figures from 2 publications |
Sequence Information
Other Construct Pages
None
Citations