Transgenic Construct
Tg(hsp70l:Hsa.IK17-EGFP)
- ID
- ZDB-TGCONSTRCT-120229-3
- Name
- Tg(hsp70l:Hsa.IK17-EGFP)
- Previous Names
- None
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:EGFP
- Contains Sequences
- Note
-
Hsa.IK17 is a human single chain monoclonal antibody against IK17. The antibody genes in IK17 are derived from several genes. The IK17 VH region is derived from the V3-23 gene rearranged to the DH, 6-06/DN4, and JH4b minigenes. The IK17 VH region is paired with the VK3 gene, Vg/L6, rearranged to JK2 (Shaw et al., Arterioscler Thromb Vasc Biol. 2001;21:1333-1339).
(hsp70:IK17-eGFP) expresses a functional single-chain antibody (scFv) of human IK17, tagged with GFP, under the control of the zebrafish hsp70 promoter. Secreted IK17-eGFP binds to malondialdehyde epitopes on lipid deposits (OxLDL and malondialdehyde-LDL) in the vascular wall. Activation of IK17-eGFP expression by heat shock results in the presence of GFP in oxidized plaques. (O'Hare et al., 2014 )
Plasmid Map
None
Genomic Features
That Utilize Tg(hsp70l:Hsa.IK17-EGFP) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| sd16Tg |
Transgenics
That Utilize Tg(hsp70l:Hsa.IK17-EGFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| sd16Tg |
|
Fig. 4 from O'Hare et al., 2014 | |
| sd16Tg (AB) |
|
Fig. 2 from Fang et al., 2011 | |
| sd16Tg + MO1-ldlra |
|
Fig. 4 from O'Hare et al., 2014 | Fig. 4 from O'Hare et al., 2014 |
Sequence Information
Other Construct Pages
None
Citations