PUBLICATION

Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development

Authors

Parichy, D.M., Rawls, J.F., Pratt, S.J., Whitfield, T.T., and Johnson, S.L.

ID

ZDB-PUB-990830-7

Date

1999

Source

Development (Cambridge, England) 126(15): 3425-3436 (Journal)

Registered Authors

Johnson, Stephen L., Parichy, David M., Pratt, Stephen J., Rawls, John F., Whitfield, Tanya T.

Keywords

neural crest; melanocyte; c-kit; Danio rerio; hematopoiesis; primordial germ cell; apoptosis; cell migration

MeSH Terms

Germ Cells/growth & development
Base Sequence
DNA, Complementary/genetics
Neural Crest/cytology
Proto-Oncogene Proteins c-kit/genetics*
Phylogeny
Species Specificity
Mice
Melanocytes/cytology*
DNA Primers/genetics
Hematopoiesis/genetics
Zebrafish/embryology*
Zebrafish/genetics*
Biological Evolution
Male
Female
Animals

(all 17)

PubMed

10393121 Full text @ Development

Abstract

The relative roles of the Kit receptor in promoting the migration and survival of amniote melanocytes are unresolved. We show that, in the zebrafish, Danio rerio, the pigment pattern mutation sparse corresponds to an orthologue of c-kit. This finding allows us to further elucidate morphogenetic roles for this c-kit-related gene in melanocyte morphogenesis. Our analyses of zebrafish melanocyte development demonstrate that the c-kit orthologue identified in this study is required both for normal migration and for survival of embryonic melanocytes. We also find that, in contrast to mouse, the zebrafish c-kit gene that we have identified is not essential for hematopoiesis or primordial germ cell development. These unexpected differences may reflect evolutionary divergence in c-kit functions following gene duplication events in teleosts.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
kita^b5/b5 (AB)	standard conditions	Pec-fin	head melanocyte absent, abnormal	Fig. 4
kita^b5/b5 (AB)	standard conditions	Pec-fin	melanocyte accumulation otic vesicle posterior-most region, abnormal	Fig. 4
kita^b5/b5 (AB)	standard conditions	Pec-fin	melanocyte accumulation pharyngeal arch lateral margin, abnormal	Fig. 4
kita^b5/b5 (AB)	standard conditions	Pec-fin	melanocyte decreased amount, abnormal	Fig. 5
kita^b5/b5 (AB)	standard conditions	Pec-fin	melanocyte migration arrested, abnormal	Fig. 4
kita^b5/b5 (AB)	standard conditions	Pec-fin	melanocyte spatial pattern, abnormal	Fig. 4
kita^b5/b5 (AB)	standard conditions	Pec-fin to Days 7-13	melanocyte apoptotic process increased occurrence, abnormal	Fig. 5
kita^b5/b5 (AB)	standard conditions	Pec-fin to Days 7-13	melanocyte decreased amount, abnormal	Fig. 5
kita^b5/b5 (AB)	standard conditions	Day 5	melanocyte apoptotic process increased occurrence, abnormal	Fig. 6
kita^b5/b5 (AB)	standard conditions	Days 7-13	melanocyte absent, abnormal	Fig. 6

1 - 10 of 13

Show

Mutations / Transgenics

Allele	Type	Affected Genomic Region
b4	Insertion	slc45a2
b5	Small Deletion	kita
b465	Deficiency	kita
j1e1	Point Mutation	kita
j1e15	Unknown	kita
j1e41	Point Mutation	kita
j1e56	Point Mutation	kita
j1g2	Deficiency	kita

1 - 8 of 8

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
Df(Chr20:kita)b465/b465
Df(Chr20:kita)j1g2/j1g2
kita^b5/b5 (AB)
kita^j1e1/j1e1
kita^j1e15
kita^j1e41/j1e41
kita^j1e56/j1e56
slc45a2^b4/b4
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Parichy et al., 1999 ZDB-PUB-990830-7 PMID:10393121

Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development

Parichy et al., 1999

ZDB-PUB-990830-7
PMID:10393121