PUBLICATION
Positive and Negative Cis-Acting Elements Are Required for Hematopoietic Expression of Zebrafish GATA-1
- Authors
- Meng, A., Tang, H., Yuan, B.Z., Ong, B.A., Long, Q.M., and Lin, S.
- ID
- ZDB-PUB-990119-23
- Date
- 1999
- Source
- Blood 93(2): 500-508 (Journal)
- Registered Authors
- Lin, Shuo, Meng, Anming, Yuan, Ben
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- DNA-Binding Proteins/genetics*
- Enhancer Elements, Genetic
- Erythroid-Specific DNA-Binding Factors
- GATA1 Transcription Factor
- Gene Deletion
- Gene Expression*
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins
- Hematopoiesis
- Hematopoietic Stem Cells/metabolism*
- Humans
- Luminescent Proteins/genetics
- Mutagenesis, Site-Directed
- Point Mutation
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Regulatory Sequences, Nucleic Acid*
- Transcription Factors/genetics*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins
- PubMed
- 9885211
Citation
Meng, A., Tang, H., Yuan, B.Z., Ong, B.A., Long, Q.M., and Lin, S. (1999) Positive and Negative Cis-Acting Elements Are Required for Hematopoietic Expression of Zebrafish GATA-1. Blood. 93(2):500-508.
Abstract
GATA-1 is a transcription factor required for development of erythroid cells. The expression of GATA-1 is tightly restricted to the hematopoietic lineage. Using transgene constructs containing zebrafish GATA-1 genomic sequences and the green fluorescent protein (GFP) reporter gene, we previously showed that a 5.6-kb enhancer/promoter fragment is sufficient to direct erythroid-specific expression of the GFP. In this study, we used enhancer/promoter fragments containing various deletion and point mutations to further characterize the cis-acting elements controlling tissue-specific GATA-1 expression. We report here the identification of distinct cis-acting elements that cooperate to confer on GATA-1 its hematopoietic expression pattern. A CACCC box, located 142 bp upstream of the translation start codon, is critical for the initiation of GATA-1 expression. A distal double GATA element is required for maintaining and enhancing the hematopoietic expression of GATA-1. The erythroid-specific activity of the GATA-1 promoter is also enhanced by a 49-bp sequence element located 218 bp upstream of the CACCC element and a CCAAT box adjacent to the double GATA motif. Finally, the hematopoietic specificity of the GATA-1 promoter is secured by a negative cis-acting element that inhibits expression in the notochord.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping