Gene mapping in zebrafish using single-strand conformation polymorphism analysis
- Förnzler, D., Her, H., Knapik, E.W., Clark, M., Lehrach, H., Postlethwait, J.H., Zon, L.I., Beier, D.R.
- Genomics 51: 216-222 (Journal)
- Registered Authors
- Beier, David R., Clark, Matthew D., Knapik, Ela W., Lehrach, Hans, Postlethwait, John H., Zon, Leonard I.
- MeSH Terms
- 3' Untranslated Regions
- Crosses, Genetic
- DNA Primers
- DNA, Complementary/genetics
- Expressed Sequence Tags
- Physical Chromosome Mapping/methods*
- Polymorphism, Single-Stranded Conformational*
- Sensitivity and Specificity
- 9722944 Full text @ Genomics
Förnzler, D., Her, H., Knapik, E.W., Clark, M., Lehrach, H., Postlethwait, J.H., Zon, L.I., Beier, D.R. (1998) Gene mapping in zebrafish using single-strand conformation polymorphism analysis. Genomics. 51:216-222.
To exploit fully the power of the zebrafish system as a model for vertebrate development, it will be necessary to develop efficient tools for genomic analysis. In this report we have tested whether single-strand conformation polymorphism analysis (SSCP) can be utilized for gene mapping in zebrafish. Over 100 primer pairs derived from noncoding regions of known genes and partially characterized cDNAs were analyzed, and a polymorphism frequency of approximately 50% was detected in zebrafish strains used for genetic mapping studies. A subset of these polymorphic cDNAs was localized on the zebrafish map. SSCP thus represents an efficient strategy for mapping transcribed sequences with a high resolution in the zebrafish genome, which will facilitate the integration of existing zebrafish framework maps, the generation of a zebrafish EST map, and the application of alternative gene localization strategies such as comparative mapping.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes