PUBLICATION
Retinoid X receptor-selective ligands produce malformations in Xenopus embryos
- Authors
- Minucci, S., Saint-Jeannet, J.P., Toyama, R., Scita, G., DeLuca, L.M., Taira, M., Levin, A.A., Ozato, K., and Dawid, I.B.
- ID
- ZDB-PUB-961014-786
- Date
- 1996
- Source
- Proceedings of the National Academy of Sciences of the United States of America 93: 1803-1807 (Journal)
- Registered Authors
- Dawid, Igor B., Ozato, Kenjiro, Toyama, Reiko
- Keywords
- none
- MeSH Terms
-
- Gene Expression Regulation, Developmental
- Ligands
- Receptors, Retinoic Acid/physiology*
- Gene Expression/drug effects
- Zebrafish/embryology*
- Animals
- Retinoids/pharmacology
- Xenopus laevis/embryology*
- Retinoid X Receptors
- Transcription Factors/physiology*
- Congenital Abnormalities/embryology
- PubMed
- 8700839 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Minucci, S., Saint-Jeannet, J.P., Toyama, R., Scita, G., DeLuca, L.M., Taira, M., Levin, A.A., Ozato, K., and Dawid, I.B. (1996) Retinoid X receptor-selective ligands produce malformations in Xenopus embryos. Proceedings of the National Academy of Sciences of the United States of America. 93:1803-1807.
Abstract
Retinoids exert pleiotropic effects on the development of vertebrates through the action of retinoic acid receptors (RAR) and retinoid X receptors (RXR). We have investigated the effect of synthetic retinoids selective for RXR and RAR on the development of Xenopus and zebrafish embryos. In Xenopus, both ligands selective for RAR and RXR caused striking malformations along the anterior-posterior axis, whereas in zebrafish only ligands specific for RAR caused embryonic malformations. In Xenopus, RAR- and RXR-selective ligands regulated the expression of the Xlim-1, gsc, and HoxA1 genes similarly as all-trans-retinoic acid. Nevertheless, RXR- selective ligands activated only an RXR responsive reporter but not an RAR responsive reporter introduced by microinjection into the Xenopus embryo, consistent with our failure to detect conversion of an RXR-selective ligand to different derivatives in the embryo. These results suggest that Xenopus embryos possess a unique response pathway in which liganded RXR can control gene expression. Our observations further illustrate the divergence in retinoid responsiveness between different vertebrate species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping