PUBLICATION

Microsatellite-centromere mapping in the zebrafish (Danio rerio)

Authors
Kauffman, E.J., Gestl, E.E., Kim, D.J., Walker, C., Hite, J.M., Yan, G., Rogan, P.K., Johnson, S.L., and Cheng, K.C.
ID
ZDB-PUB-961014-537
Date
1995
Source
Genomics   30: 337-341 (Journal)
Registered Authors
Cheng, Keith C., Gestl, Erin, Johnson, Stephen L., Kauffman, Elizabeth J., Walker, Charline
Keywords
none
MeSH Terms
  • Animals
  • Centromere*
  • DNA, Satellite/genetics*
  • Female
  • Heterozygote
  • Homozygote
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
8586435 Full text @ Genomics
Abstract
Ten (CA)n microsatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half- tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P < 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping