Microsatellite-centromere mapping in the zebrafish (Danio rerio)
- Kauffman, E.J., Gestl, E.E., Kim, D.J., Walker, C., Hite, J.M., Yan, G., Rogan, P.K., Johnson, S.L., and Cheng, K.C.
- Genomics 30: 337-341 (Journal)
- Registered Authors
- Cheng, Keith C., Gestl, Erin, Johnson, Stephen L., Kauffman, Elizabeth J., Walker, Charline
- MeSH Terms
- DNA, Satellite/genetics*
- 8586435 Full text @ Genomics
Kauffman, E.J., Gestl, E.E., Kim, D.J., Walker, C., Hite, J.M., Yan, G., Rogan, P.K., Johnson, S.L., and Cheng, K.C. (1995) Microsatellite-centromere mapping in the zebrafish (Danio rerio). Genomics. 30:337-341.
Ten (CA)n microsatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half- tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P < 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes