PUBLICATION
Complex expression of the zp-50 pou gene in the embryonic zebrafish brain is altered by overexpression of sonic hedgehog
- Authors
- Hauptmann, G. and Gerster, T.
- ID
- ZDB-PUB-961014-426
- Date
- 1996
- Source
- Development (Cambridge, England) 122(6): 1769-1780 (Journal)
- Registered Authors
- Gerster, Thomas, Hauptmann, Giselbert
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/embryology*
- Brain/metabolism
- Cloning, Molecular
- DNA
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Hedgehog Proteins
- Mesencephalon/metabolism
- Molecular Sequence Data
- Mutation
- Nerve Tissue Proteins/biosynthesis
- Nerve Tissue Proteins/genetics*
- POU Domain Factors
- Prosencephalon/metabolism
- Proteins/genetics
- Proteins/physiology*
- Rhombencephalon/metabolism
- Sequence Homology, Amino Acid
- Trans-Activators*
- Transcription Factors/biosynthesis
- Transcription Factors/genetics*
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 8674416 Full text @ Development
Citation
Hauptmann, G. and Gerster, T. (1996) Complex expression of the zp-50 pou gene in the embryonic zebrafish brain is altered by overexpression of sonic hedgehog. Development (Cambridge, England). 122(6):1769-1780.
Abstract
We report the characterization of the zebrafish zp-50 class III POU domain gene. This gene is first activated in the prospective diencephalon after the end of the gastrula period. During somitogenesis, zp-50 is expressed in a very dynamic and complex fashion in all major subdivisions of the central nervous system. After one day of development, zp-50 transcripts are present in the fore- and midbrain in several distinct cell clusters. In the hindbrain, zp-50 expression is found in two types of domains. Correct zp-50 expression in the ventral fore- and midbrain requires genes known to be involved in dorsoventral patterning of the zebrafish CNS. Transcripts of the sonic hedgehog (shh) gene encoding an intercellular signaling molecule are detected in the forming diencephalon shortly prior to the appearance of zp-50 mRNA. Correct expression in this region of both shh, and zp-50, requires a functional cyclops (cyc) locus: shh and zp-50 transcripts are likewise absent from the ventral rostral brain of mutant cyc-/- embryos. Injection of synthetic shh mRNA into fertilized eggs causes ectopic zp-50 expression at more dorsal positions of the embryonic brain. The close spatial and temporal coincidence of expression in the rostral brain, the similar response to the cyc- mutation, and the ectopic zp-50 expression in the injection experiments all suggest that zp-50 may directly respond to the reception of the Shh signal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping