PUBLICATION

Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos

Authors
Thisse, C., Thisse, B., Schilling, T.F., and Postlethwait, J.H.
ID
ZDB-PUB-961014-1118
Date
1993
Source
Development (Cambridge, England)   119: 1203-1215 (Journal)
Registered Authors
Postlethwait, John H., Schilling, Tom, Thisse, Bernard, Thisse, Christine
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gastrula/physiology
  • Gene Expression/genetics*
  • Genes/genetics*
  • In Situ Hybridization
  • Mesoderm/physiology
  • Molecular Sequence Data
  • Morphogenesis/genetics
  • Mutation/genetics
  • Neural Crest/physiology
  • Sequence Homology
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
8306883 Full text @ Development
Abstract
Mesoderm formation is critical for the establishment of the animal body plan and in Drosophila requires the snail gene. This report concerns the cloning and expression pattern of the structurally similar gene snail1 from zebrafish. In situ hybridization shows that the quantity of snail1 RNA increases at the margin of the blastoderm in cells that involute during gastrulation. As gastrulation begins, snail1 RNA disappears from the dorsal axial mesoderm and becomes restricted to the paraxial mesoderm and the tail bud. snail1 RNA increases in cells that define the posterior border of each somite and then disappears when somitic cells differentiate. Later in development, expression appears in cephalic neural crest derivatives. Many snail1-expressing cells were missing from mutant spadetail embryos and the quantity of snail1 RNA was greatly reduced in mutant no tail embryos. The work presented here suggests that snail1 is involved in morphogenetic events during gastrulation, somitogenesis and development of the cephalic neural crest, and that no tail may act as a positive regulator of snail1.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes