PUBLICATION
The Zpr-3 Antibody Recognizes the 320-354 Region of Rho and Labels Both Rods and Green Cones in Zebrafish
- Authors
- Hu, H., Qin, Y., Qu, Z., Huang, Y., Ren, X., Liu, M., Liu, F., Gao, P.
- ID
- ZDB-PUB-240925-3
- Date
- 2024
- Source
- Zebrafish 21(6): 394-400 (Journal)
- Registered Authors
- Huang, Yuwen, Liu, Fei, Liu, Mugen, Qin, Yayun, Qu, Zhen
- Keywords
- Zpr-3, green cone, retina, rhodopsin, rod, zebrafish
- MeSH Terms
-
- Animals
- Zebrafish*/immunology
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/immunology
- Zebrafish Proteins*/metabolism
- Rhodopsin/immunology
- Rhodopsin/metabolism
- Retinal Cone Photoreceptor Cells*/metabolism
- Retinal Rod Photoreceptor Cells/metabolism
- Antibodies/immunology
- PubMed
- 39316468 Full text @ Zebrafish
Citation
Hu, H., Qin, Y., Qu, Z., Huang, Y., Ren, X., Liu, M., Liu, F., Gao, P. (2024) The Zpr-3 Antibody Recognizes the 320-354 Region of Rho and Labels Both Rods and Green Cones in Zebrafish. Zebrafish. 21(6):394-400.
Abstract
Retinal markers with high quality and specificity are important for the observation of pathologic changes of retinal cells during retinal development, degeneration, and regeneration. The zpr-3 antibody is widely used to label rods in zebrafish, but the exact antigen is still unknown. In this study, we provided evidence to demonstrate that the antigen gene of zpr-3 is rho, which encodes the rod opsin, and the exact epitope of zpr-3 is the 320-354 region of Rho protein. More importantly, our immunofluorescence assays indicated that zpr-3 labels both the outer segments of rods and green cones on zebrafish retinal sections, probably due to the cross-reaction with the green-cone opsin. Our work is valuable for the scientific community to interpret the experimental data involving the zpr-3 antibody.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping