PUBLICATION
Mycb and Mych stimulate Müller glial cell reprogramming and proliferation in the uninjured and injured zebrafish retina
- Authors
- Lee, M.S., Jui, J., Sahu, A., Goldman, D.
- ID
- ZDB-PUB-240711-2
- Date
- 2024
- Source
- Development (Cambridge, England) 151(14): (Journal)
- Registered Authors
- Goldman, Dan
- Keywords
- Apoptosis, Cell cycle, Myc, Protein synthesis, Regeneration, Stem cell
- Datasets
- GEO:GSE249115, GEO:GSE249117, GEO:GSE249116
- MeSH Terms
-
- Apoptosis/genetics
- Cellular Reprogramming*/genetics
- Zebrafish*
- Transcriptome/genetics
- Ependymoglial Cells*/cytology
- Ependymoglial Cells*/metabolism
- Cell Proliferation*
- Retina*/cytology
- Retina*/metabolism
- Retinal Neurons/metabolism
- Animals
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 38984586 Full text @ Development
Citation
Lee, M.S., Jui, J., Sahu, A., Goldman, D. (2024) Mycb and Mych stimulate Müller glial cell reprogramming and proliferation in the uninjured and injured zebrafish retina. Development (Cambridge, England). 151(14):.
Abstract
In the injured zebrafish retina, Müller glial cells (MG) reprogram to adopt retinal stem cell properties and regenerate damaged neurons. The strongest zebrafish reprogramming factors might be good candidates for stimulating a similar regenerative response by mammalian MG. Myc proteins are potent reprogramming factors that can stimulate cellular plasticity in differentiated cells; however, their role in MG reprogramming and retina regeneration remains poorly explored. Here we report that retinal injury stimulates mycb and mych expression and that although both Mycb and Mych stimulate MG reprogramming and proliferation, only Mych enhances retinal neuron apoptosis. RNAseq analysis of Wt, mychmut, and mycbmut fish revealed Mycb and Mych regulate ∼40% and ∼16%, respectively, of the genes contributing to MG's regeneration-associated transcriptome. Of these genes, those that are induced are biased towards regulating ribosome biogenesis, protein synthesis, DNA synthesis, and cell division which are the top cellular processes regulated by retinal injury and this suggests Mycb and Mych are potent MG reprogramming factors. Consistent with this, forced expression of either of these proteins is sufficient to stimulate MG proliferation in the uninjured retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping