PUBLICATION
scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation
- Authors
- Abu Nahia, K., Sulej, A., Migdał, M., Ochocka, N., Ho, R., Kamińska, B., Zagorski, M., Winata, C.L.
- ID
- ZDB-PUB-240614-9
- Date
- 2024
- Source
- iScience 27: 110083110083 (Journal)
- Registered Authors
- Abu Nahia, Karim, Sulej, Agata, Winata, Cecilia Lanny
- Keywords
- Embryology, Integrative aspects of cell biology, Model organism, Transcriptomics
- Datasets
- GEO:GSE234216
- MeSH Terms
- none
- PubMed
- 38872974 Full text @ iScience
Citation
Abu Nahia, K., Sulej, A., Migdał, M., Ochocka, N., Ho, R., Kamińska, B., Zagorski, M., Winata, C.L. (2024) scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation. iScience. 27:110083110083.
Abstract
We utilized scRNA-seq to delineate the diversity of cell types in the zebrafish heart. Transcriptome profiling of over 50,000 cells at 48 and 72 hpf defined at least 18 discrete cell lineages of the developing heart. Utilizing well-established gene signatures, we identified a population of cells likely to be the primary pacemaker and characterized the transcriptome profile defining this critical cell type. Two previously uncharacterized genes, atp1b3b and colec10, were found to be enriched in the sinoatrial cardiomyocytes. CRISPR/Cas9-mediated knockout of these two genes significantly reduced heart rate, implicating their role in cardiac development and conduction. Additionally, we describe other cardiac cell lineages, including the endothelial and neural cells, providing their expression profiles as a resource. Our results established a detailed atlas of the developing heart, providing valuable insights into cellular and molecular mechanisms, and pinpointed potential new players in heart rhythm regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping