PUBLICATION
Extremely low-frequency electromagnetic fields facilitate both osteoblast and osteoclast activity through Wnt/β-catenin signaling in the zebrafish scale
- Authors
- Kobayashi-Sun, J., Kobayashi, I., Kashima, M., Hirayama, J., Kakikawa, M., Yamada, S., Suzuki, N.
- ID
- ZDB-PUB-240222-32
- Date
- 2024
- Source
- Frontiers in cell and developmental biology 12: 13400891340089 (Journal)
- Registered Authors
- Hirayama, Jun, Kobayashi, Isao
- Keywords
- Wnt/β-catenin signaling, electromagnetic fields, osteoblast, osteoclast, zebrafish scale
- Datasets
- GEO:GSE247669
- MeSH Terms
- none
- PubMed
- 38385024 Full text @ Front Cell Dev Biol
Citation
Kobayashi-Sun, J., Kobayashi, I., Kashima, M., Hirayama, J., Kakikawa, M., Yamada, S., Suzuki, N. (2024) Extremely low-frequency electromagnetic fields facilitate both osteoblast and osteoclast activity through Wnt/β-catenin signaling in the zebrafish scale. Frontiers in cell and developmental biology. 12:13400891340089.
Abstract
Electromagnetic fields (EMFs) have received widespread attention as effective, noninvasive, and safe therapies across a range of clinical applications for bone disorders. However, due to the various frequencies of devices, their effects on tissues/cells are vary, which has been a bottleneck in understanding the effects of EMFs on bone tissue. Here, we developed an in vivo model system using zebrafish scales to investigate the effects of extremely low-frequency EMFs (ELF-EMFs) on fracture healing. Exposure to 10 millitesla (mT) of ELF-EMFs at 60 Hz increased the number of both osteoblasts and osteoclasts in the fractured scale, whereas 3 or 30 mT did not. Gene expression analysis revealed that exposure to 10 mT ELF-EMFs upregulated wnt10b and Wnt target genes in the fractured scale. Moreover, β-catenin expression was enhanced by ELF-EMFs predominantly at the fracture site of the zebrafish scale. Inhibition of Wnt/β-catenin signaling by IWR-1-endo treatment reduced both osteoblasts and osteoclasts in the fractured scale exposed to ELF-EMFs. These results suggest that ELF-EMFs promote both osteoblast and osteoclast activity through activation of Wnt/β-catenin signaling in fracture healing. Our data provide in vivo evidence that ELF-EMFs generated with a widely used commercial AC power supply have a facilitative effect on fracture healing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping