PUBLICATION
Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator
- Authors
- Knabl, P., Schauer, A., Pomreinke, A.P., Zimmermann, B., Rogers, K.W., Čapek, D., Müller, P., Genikhovich, G.
- ID
- ZDB-PUB-240208-3
- Date
- 2024
- Source
- eLIFE 13: (Journal)
- Registered Authors
- Müller, Patrick
- Keywords
- BMP signaling, Bilateria, Cnidaria, Danio, Nematostella, developmental biology, evolution of axial patterning, evolutionary biology, nematostella vectensis, zebrafish
- MeSH Terms
-
- Animals
- Body Patterning/genetics
- Gene Expression
- Gene Expression Regulation, Developmental
- Genome
- Sea Anemones*/genetics
- Signal Transduction
- PubMed
- 38323609 Full text @ Elife
Citation
Knabl, P., Schauer, A., Pomreinke, A.P., Zimmermann, B., Rogers, K.W., Čapek, D., Müller, P., Genikhovich, G. (2024) Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. eLIFE. 13:.
Abstract
BMP signaling has a conserved function in patterning the dorsal-ventral body axis in Bilateria and the directive axis in anthozoan cnidarians. So far, cnidarian studies have focused on the role of different BMP signaling network components in regulating pSMAD1/5 gradient formation. Much less is known about the target genes downstream of BMP signaling. To address this, we generated a genome-wide list of direct pSMAD1/5 target genes in the anthozoan Nematostella vectensis, several of which were conserved in Drosophila and Xenopus. Our ChIP-seq analysis revealed that many of the regulatory molecules with documented bilaterally symmetric expression in Nematostella are directly controlled by BMP signaling. We identified several so far uncharacterized BMP-dependent transcription factors and signaling molecules, whose bilaterally symmetric expression may be indicative of their involvement in secondary axis patterning. One of these molecules is zswim4-6, which encodes a novel nuclear protein that can modulate the pSMAD1/5 gradient and potentially promote BMP-dependent gene repression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping