PUBLICATION
Definitive hematopoiesis is dispensable to sustain erythrocytes and macrophages during zebrafish ontogeny
- Authors
- Elsaid, R., Mikdache, A., Castillo, K.Q., Salloum, Y., Diabangouaya, P., Gros, G., Feijoo, C.G., Hernández, P.P.
- ID
- ZDB-PUB-240208-16
- Date
- 2024
- Source
- iScience 27: 108922108922 (Journal)
- Registered Authors
- Diabangouaya, Patricia, Feijoo, Carmen G., Hernández-Cerda, Pedro
- Keywords
- Biological sciences, Developmental biology, Embryology
- MeSH Terms
- none
- PubMed
- 38327794 Full text @ iScience
Citation
Elsaid, R., Mikdache, A., Castillo, K.Q., Salloum, Y., Diabangouaya, P., Gros, G., Feijoo, C.G., Hernández, P.P. (2024) Definitive hematopoiesis is dispensable to sustain erythrocytes and macrophages during zebrafish ontogeny. iScience. 27:108922108922.
Abstract
In all organisms studied, from flies to humans, blood cells emerge in several sequential waves and from distinct hematopoietic origins. However, the relative contribution of these ontogenetically distinct hematopoietic waves to embryonic blood lineages and to tissue regeneration during development is yet elusive. Here, using a lineage-specific "switch and trace" strategy in the zebrafish embryo, we report that the definitive hematopoietic progeny barely contributes to erythrocytes and macrophages during early development. Lineage tracing further shows that ontogenetically distinct macrophages exhibit differential recruitment to the site of injury based on the developmental stage of the organism. We further demonstrate that primitive macrophages can solely maintain tissue regeneration during early larval developmental stages after selective ablation of definitive macrophages. Our findings highlight that the sequential emergence of hematopoietic waves in embryos ensures the abundance of blood cells required for tissue homeostasis and integrity during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping