PUBLICATION
A vibrating capillary for ultrasound rotation manipulation of zebrafish larvae
- Authors
- Zhang, Z., Cao, Y., Caviglia, S., Agrawal, P., Neuhauss, S.C.F., Ahmed, D.
- ID
- ZDB-PUB-240110-12
- Date
- 2024
- Source
- Lab on a Chip 24(4): 764-775 (Journal)
- Registered Authors
- Neuhauss, Stephan
- Keywords
- none
- MeSH Terms
-
- Animals
- Larva
- Micromanipulation*
- Rotation
- Zebrafish*
- PubMed
- 38193588 Full text @ Lab Chip
Citation
Zhang, Z., Cao, Y., Caviglia, S., Agrawal, P., Neuhauss, S.C.F., Ahmed, D. (2024) A vibrating capillary for ultrasound rotation manipulation of zebrafish larvae. Lab on a Chip. 24(4):764-775.
Abstract
Multifunctional micromanipulation systems have garnered significant attention due to the growing interest in biological and medical research involving model organisms like zebrafish (Danio rerio). Here, we report a novel acoustofluidic rotational micromanipulation system that offers rapid trapping, high-speed rotation, multi-angle imaging, and 3D model reconstruction of zebrafish larvae. An ultrasound-activated oscillatory glass capillary is used to trap and rotate a zebrafish larva. Simulation and experimental results demonstrate that both the vibrating mode and geometric placement of the capillary contribute to the developed polarized vortices along the long axis of the capillary. Given its capacities for easy-to-operate, stable rotation, avoiding overheating, and high-throughput manipulation, our system poses the potential to accelerate zebrafish-directed biomedical research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping