PUBLICATION
Common and divergent gene regulatory networks control injury-induced and developmental neurogenesis in zebrafish retina
- Authors
- Lyu, P., Iribarne, M., Serjanov, D., Zhai, Y., Hoang, T., Campbell, L.J., Boyd, P., Palazzo, I., Nagashima, M., Silva, N.J., Hitchcock, P.F., Qian, J., Hyde, D.R., Blackshaw, S.
- ID
- ZDB-PUB-231221-11
- Date
- 2023
- Source
- Nature communications 14: 84778477 (Journal)
- Registered Authors
- Campbell, Leah, Hitchcock, Peter, Hyde, David R., Nagashima, Mikiko
- Keywords
- none
- Datasets
- GEO:GSE135406, GEO:GSE239410
- MeSH Terms
-
- Neurogenesis/genetics
- Retina/metabolism
- Neuroglia/metabolism
- Cell Proliferation/physiology
- Gene Regulatory Networks*
- Animals
- Zebrafish*/genetics
- Ependymoglial Cells/metabolism
- PubMed
- 38123561 Full text @ Nat. Commun.
Citation
Lyu, P., Iribarne, M., Serjanov, D., Zhai, Y., Hoang, T., Campbell, L.J., Boyd, P., Palazzo, I., Nagashima, M., Silva, N.J., Hitchcock, P.F., Qian, J., Hyde, D.R., Blackshaw, S. (2023) Common and divergent gene regulatory networks control injury-induced and developmental neurogenesis in zebrafish retina. Nature communications. 14:84778477.
Abstract
Following acute retinal damage, zebrafish possess the ability to regenerate all neuronal subtypes through Müller glia (MG) reprogramming and asymmetric cell division that produces a multipotent Müller glia-derived neuronal progenitor cell (MGPC). This raises three key questions. First, do MG reprogram to a developmental retinal progenitor cell (RPC) state? Second, to what extent does regeneration recapitulate retinal development? And finally, does loss of different retinal cell subtypes induce unique MG regeneration responses? We examined these questions by performing single-nuclear and single-cell RNA-Seq and ATAC-Seq in both developing and regenerating retinas. Here we show that injury induces MG to reprogram to a state similar to late-stage RPCs. However, there are major transcriptional differences between MGPCs and RPCs, as well as major transcriptional differences between activated MG and MGPCs when different retinal cell subtypes are damaged. Validation of candidate genes confirmed that loss of different subtypes induces differences in transcription factor gene expression and regeneration outcomes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping