PUBLICATION
Analysis of sleep/wake cycles in zebrafish larvae
- Authors
- Ricarte, M., Prats, E., Bedrossiantz, J., Raldúa, D.
- ID
- ZDB-PUB-231214-4
- Date
- 2023
- Source
- MethodsX 12: 102492102492 (Journal)
- Registered Authors
- Raldúa, Demetrio
- Keywords
- Analysis of sleep/wake cycles in zebrafish larvae, Sleep disturbance, Sleep/wake cycles, Video-tracking technologies, Zebrafish larvae
- MeSH Terms
- none
- PubMed
- 38089153 Full text @ MethodsX
Citation
Ricarte, M., Prats, E., Bedrossiantz, J., Raldúa, D. (2023) Analysis of sleep/wake cycles in zebrafish larvae. MethodsX. 12:102492102492.
Abstract
Zebrafish larvae are a model organism increasingly used in the study of the effect of neuroactive chemicals on vertebrate sleep/wake cycles. Sleep disturbances have a negative impact on mood, cognition and overall health. Here we present a protocol to assess over 24 h sleep/wake cycles in zebrafish larvae subjected to 12 h light/dark periods in 48-well plates, using video-tracking technologies. The protocol can be used to determine if the exposure to environmental pollutants or drugs can lead to sleep disturbances. The results on the effect of the tire rubber-derived 6PPD-quinone on zebrafish sleep/wake cycles presented here demonstrate the suitability of using this protocol in fish neurotoxicity studies. This protocol provides a new relevant tool to be used in the pharmacology and toxicology fields.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping