PUBLICATION
Transmembrane protein 14A protects glomerular filtration barrier integrity
- Authors
- Khalil, R., Bonnemaijer, J.D.D., Kreutz, R., Spaink, H.P., Hogendoorn, P.C.W., Baelde, H.J.
- ID
- ZDB-PUB-231207-1
- Date
- 2023
- Source
- Physiological Reports 11: e15847e15847 (Journal)
- Registered Authors
- Spaink, Herman P.
- Keywords
- TMEM14A, cell, glomerulus, kidney, podocyte, proteinuria, rat, zebrafish
- MeSH Terms
-
- Animals
- Glomerular Filtration Barrier*
- Kidney Glomerulus/metabolism
- Podocytes*/metabolism
- Proteinuria/metabolism
- Rats
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 38054547 Full text @ Physiol. Rep.
Citation
Khalil, R., Bonnemaijer, J.D.D., Kreutz, R., Spaink, H.P., Hogendoorn, P.C.W., Baelde, H.J. (2023) Transmembrane protein 14A protects glomerular filtration barrier integrity. Physiological Reports. 11:e15847e15847.
Abstract
Transmembrane protein 14A (TMEM14A) is a relatively unknown protein that is now identified to be required for maintaining the integrity of the glomerular filtration barrier. It is an integral transmembrane protein of 99 amino acids with three transmembrane domains. TMEM14A has been implied to suppress Bax-mediated apoptosis in other studies. Other than that, little is currently known of its function. Here, we show that its expression is diminished before onset of proteinuria in a spontaneously proteinuric rat model. Knocking down tmem14a mRNA translation results in proteinuria in zebrafish embryos without affecting tubular reabsorption. Also, it is primarily expressed by podocytes. Lastly, an increase in glomerular TMEM14A expression is exhibited in various proteinuric renal diseases. Overall, these results suggest that TMEM14A is a novel factor in the protective mechanisms of the nephron to maintain glomerular filtration barrier integrity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping