PUBLICATION
Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling
- Authors
- Belmonte-Mateos, C., Meister, L., Pujades, C.
- ID
- ZDB-PUB-231130-8
- Date
- 2023
- Source
- Frontiers in cell and developmental biology 11: 12686311268631 (Journal)
- Registered Authors
- Belmonte-Mateos, Carla, Pujades, Cristina
- Keywords
- Notch signaling, cell division mode, hindbrain rhombomeres, neural progenitors, neurogenesis, zebrafish
- MeSH Terms
- none
- PubMed
- 38020924 Full text @ Front Cell Dev Biol
Citation
Belmonte-Mateos, C., Meister, L., Pujades, C. (2023) Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling. Frontiers in cell and developmental biology. 11:12686311268631.
Abstract
Tissue growth and morphogenesis are interrelated processes, whose tight coordination is essential for the production of different cell fates and the timely precise allocation of stem cell capacities. The zebrafish embryonic brainstem, the hindbrain, exemplifies such coupling between spatiotemporal cell diversity acquisition and tissue growth as the neurogenic commitment is differentially distributed over time. Here, we combined cell lineage and in vivo imaging approaches to reveal the emergence of specific cell population properties within the rhombomeres. We studied the molecular identity of hindbrain rhombomere centers and showed that they harbor different progenitor capacities that change over time. By clonal analysis, we revealed that cells within the center of rhombomeres decrease the proliferative capacity to remain mainly in the G1 phase. Proliferating progenitors give rise to neurons by asymmetric and symmetric neurogenic divisions while maintaining the pool of progenitors. The proliferative capacity of these cells differs from their neighbors, and they are delayed in the onset of Notch activity. Through functional studies, we demonstrated that they rely on Notch3 signaling to be maintained as non-committed progenitors. In this study, we show that cells in rhombomere centers, despite the neurogenic asynchrony, might share steps of a similar program with the rhombomere counterparts, to ensure proper tissue growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping