PUBLICATION
In toto imaging of glial JNK signaling during larval zebrafish spinal cord regeneration
- Authors
- Becker, C.J., Cigliola, V., Gillotay, P., Rich, A., De Simone, A., Han, Y., Di Talia, S., Poss, K.D.
- ID
- ZDB-PUB-231124-12
- Date
- 2023
- Source
- Development (Cambridge, England) 150(24): (Journal)
- Registered Authors
- Becker, Clay, Cigliola, Valentina, Gillotay, Pierre, Han, Yanchao, Poss, Kenneth D., Rich, Ashley
- Keywords
- JNK Signaling, Kinase translocation reporter, Regeneration, Spinal cord, Zebrafish
- MeSH Terms
-
- Nerve Regeneration/physiology
- Spinal Cord
- Neuroglia/physiology
- Spinal Cord Regeneration*
- Zebrafish/physiology
- JNK Mitogen-Activated Protein Kinases
- Animals
- Spinal Cord Injuries*
- Mammals
- Larva
- PubMed
- 37997694 Full text @ Development
Citation
Becker, C.J., Cigliola, V., Gillotay, P., Rich, A., De Simone, A., Han, Y., Di Talia, S., Poss, K.D. (2023) In toto imaging of glial JNK signaling during larval zebrafish spinal cord regeneration. Development (Cambridge, England). 150(24):.
Abstract
Identification of signaling events that contribute to innate spinal cord regeneration in zebrafish can inform new targets for modulating injury responses of the mammalian central nervous system. Using a chemical screen, we identify JNK signaling as a necessary regulator of glial cell cycling and tissue bridging during spinal cord regeneration in larval zebrafish. With a kinase translocation reporter, we visualize and quantify JNK signaling dynamics at single-cell resolution in glial cell populations in developing larvae and during injury-induced regeneration. Glial JNK signaling is patterned in time and space during development and regeneration, decreasing globally as the tissue matures and increasing in the rostral cord stump upon transection injury. Thus, dynamic and regional regulation of JNK signaling help to direct glial cell behaviors during innate spinal cord regeneration.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping