PUBLICATION
Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regeneration
- Authors
- Celotto, L., Rost, F., Machate, A., Bläsche, J., Dahl, A., Weber, A., Hans, S., Brand, M.
- ID
- ZDB-PUB-231122-15
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Brand, Michael, Hans, Stefan, Machate, Anja
- Keywords
- developmental biology, light lesion, progenitors, regeneration, retina, scRNAseq, zebrafish
- Datasets
- GEO:GSE226373
- MeSH Terms
-
- Animals
- Cell Proliferation
- Gene Regulatory Networks*
- Humans
- Nerve Regeneration/genetics
- Neuroglia/metabolism
- Retina/physiology
- Sequence Analysis, RNA
- Zebrafish*/metabolism
- PubMed
- 37988404 Full text @ Elife
Citation
Celotto, L., Rost, F., Machate, A., Bläsche, J., Dahl, A., Weber, A., Hans, S., Brand, M. (2023) Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regeneration. eLIFE. 12:.
Abstract
In the lesioned zebrafish retina, Müller glia produce multipotent retinal progenitors that generate all retinal neurons, replacing lost cell types. To study the molecular mechanisms linking Müller glia reactivity to progenitor production and neuronal differentiation, we used single-cell RNA sequencing of Müller glia, progenitors and regenerated progeny from uninjured and light-lesioned retinae. We discover an injury-induced Müller glia differentiation trajectory that leads into a cell population with a hybrid identity expressing marker genes of Müller glia and progenitors. A glial self-renewal and a neurogenic trajectory depart from the hybrid cell population. We further observe that neurogenic progenitors progressively differentiate to generate retinal ganglion cells first and bipolar cells last, similar to the events observed during retinal development. Our work provides a comprehensive description of Müller glia and progenitor transcriptional changes and fate decisions in the regenerating retina, which are key to tailor cell differentiation and replacement therapies for retinal dystrophies in humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping