PUBLICATION
Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection
- Authors
- Tazin, N., Lambert, C.J., Samuel, R., Stevenson, T.J., Bonkowsky, J.L., Gale, B.K.
- ID
- ZDB-PUB-231017-57
- Date
- 2023
- Source
- Biotechnology reports (Amsterdam, Netherlands) 40: e00814e00814 (Journal)
- Registered Authors
- Bonkowsky, Joshua
- Keywords
- DNA, Electroporation, Gene delivery, Microinjection, Zebrafish
- MeSH Terms
- none
- PubMed
- 37840570 Full text @ Biotechnol Rep (Amst)
Citation
Tazin, N., Lambert, C.J., Samuel, R., Stevenson, T.J., Bonkowsky, J.L., Gale, B.K. (2023) Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection. Biotechnology reports (Amsterdam, Netherlands). 40:e00814e00814.
Abstract
Electroporation is regularly used to deliver agents into cells, including transgenic materials, but it is not used for mutating zebrafish embryos due to the lack of suitable systems, information on appropriate operating parameters, and the challenges posed by the protective chorion. Here, a novel method for gene delivery in zebrafish embryos was developed by combining microinjection into the space between the chorion and the embryo followed by electroporation. This method eliminates the need for chorion removal and injecting into the space between the chorion and embryo eliminates the need for finding and identifying key cell locations before performing an injection, making the process much simpler and more automatable. We also developed a microfluidic electroporation system and optimized electric pulse parameters for transgenesis of embryos. The study provided a novel method for gene delivery in zebrafish embryos that can be potentially implemented in a high throughput transgenesis or mutagenesis system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping