PUBLICATION
A role for dopamine in control of the hypoxic ventilatory response via D2 receptors in the zebrafish gill
- Authors
- Reed, M., Pan, W., Musa, L., Arlotta, S., Mennigen, J.A., Jonz, M.G.
- ID
- ZDB-PUB-231015-69
- Date
- 2023
- Source
- The Journal of comparative neurology 532(2): e25548 (Journal)
- Registered Authors
- Jonz, Michael G.
- Keywords
- NEC, dopamine active transporter, gill, hypoxia, tyrosine hydroxylase, zebrafish
- MeSH Terms
-
- Animals
- Dopamine/metabolism
- Gills*
- Hypoxia/metabolism
- Larva/metabolism
- Oxygen
- Zebrafish*/metabolism
- PubMed
- 37837632 Full text @ J. Comp. Neurol.
Citation
Reed, M., Pan, W., Musa, L., Arlotta, S., Mennigen, J.A., Jonz, M.G. (2023) A role for dopamine in control of the hypoxic ventilatory response via D2 receptors in the zebrafish gill. The Journal of comparative neurology. 532(2):e25548.
Abstract
Dopamine is a neurotransmitter involved in oxygen sensing and control of reflex hyperventilation. In aquatic vertebrates, oxygen sensing occurs in the gills via chemoreceptive neuroepithelial cells (NECs), but a mechanism for dopamine in autonomic control of ventilation has not been defined. We used immunohistochemistry and confocal microscopy to map the distribution of tyrosine hydroxylase (TH), an enzyme necessary for dopamine synthesis, in the gills of zebrafish. TH was found in nerve fibers of the gill filaments and respiratory lamellae. We further identified dopamine active transporter (dat) and vesicular monoamine transporter (vmat2) expression in neurons of the gill filaments using transgenic lines. Moreover, TH- and dat-positive nerve fibers innervated NECs. In chemical screening assays, domperidone, a D2 receptor antagonist, increased ventilation frequency in zebrafish larvae in a dose-dependent manner. When larvae were confronted with acute hypoxia, the D2 agonist, quinpirole, abolished the hyperventilatory response. Quantitative polymerase chain reaction confirmed expression of drd2a and drd2b (genes encoding D2 receptors) in the gills, and their relative abundance decreased following acclimation to hypoxia for 48 h. We localized D2 receptor immunoreactivity to NECs in the efferent gill filament epithelium, and a novel cell type in the afferent filament epithelium. We provide evidence for the synthesis and storage of dopamine by sensory nerve terminals that innervate NECs. We further suggest that D2 receptors on presynaptic NECs provide a feedback mechanism that attenuates the chemoreceptor response to hypoxia. Our studies suggest that a fundamental, modulatory role for dopamine in oxygen sensing arose early in vertebrate evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping