PUBLICATION
Determinants of motor neuron functional subtypes important for locomotor speed
- Authors
- D'Elia, K.P., Hameedy, H., Goldblatt, D., Frazel, P., Kriese, M., Zhu, Y., Hamling, K.R., Kawakami, K., Liddelow, S.A., Schoppik, D., Dasen, J.S.
- ID
- ZDB-PUB-230908-53
- Date
- 2023
- Source
- Cell Reports 42: 113049113049 (Journal)
- Registered Authors
- Kawakami, Koichi, Schoppik, David, Zhu, Yunlu
- Keywords
- CP: Developmental biology, CP: Neuroscience, locomotion, motor neuron, neural development, neural subtype, specification, spinal cord, transcription factor, zebrafish
- Datasets
- GEO:GSE240026
- MeSH Terms
-
- Animals
- Locomotion
- Motor Neurons/physiology
- Spinal Cord*
- Transcription Factors/genetics
- Zebrafish*
- PubMed
- 37676768 Full text @ Cell Rep.
Citation
D'Elia, K.P., Hameedy, H., Goldblatt, D., Frazel, P., Kriese, M., Zhu, Y., Hamling, K.R., Kawakami, K., Liddelow, S.A., Schoppik, D., Dasen, J.S. (2023) Determinants of motor neuron functional subtypes important for locomotor speed. Cell Reports. 42:113049113049.
Abstract
Locomotion requires precise control of the strength and speed of muscle contraction and is achieved by recruiting functionally distinct subtypes of motor neurons (MNs). MNs are essential to movement and differentially susceptible in disease, but little is known about how MNs acquire functional subtype-specific features during development. Using single-cell RNA profiling in embryonic and larval zebrafish, we identify novel and conserved molecular signatures for MN functional subtypes and identify genes expressed in both early post-mitotic and mature MNs. Assessing MN development in genetic mutants, we define a molecular program essential for MN functional subtype specification. Two evolutionarily conserved transcription factors, Prdm16 and Mecom, are both functional subtype-specific determinants integral for fast MN development. Loss of prdm16 or mecom causes fast MNs to develop transcriptional profiles and innervation similar to slow MNs. These results reveal the molecular diversity of vertebrate axial MNs and demonstrate that functional subtypes are specified through intrinsic transcriptional codes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping