PUBLICATION
Anti-HER2 Super Stealth Immunoliposomes for Targeted-Chemotherapy
- Authors
- Canato, E., Grigoletto, A., Zanotto, I., Tedeschini, T., Campara, B., Quaglio, G., Toffoli, G., Mandracchia, D., Dinarello, A., Tiso, N., Argenton, F., Sayaf, K., Guido, M., Gabbia, D., De Martin, S., Pasut, G.
- ID
- ZDB-PUB-230818-46
- Date
- 2023
- Source
- Advanced Healthcare Materials 12(29): e2301650 (Journal)
- Registered Authors
- Argenton, Francesco, Tiso, Natascia
- Keywords
- anticancer therapy, doxorubicin, drug targeting, liposome, targeted chemotherapy, trastuzumab
- MeSH Terms
-
- Animals
- Drug Delivery Systems
- Liposomes*/chemistry
- Neoplasms*
- Phospholipids
- Polyethylene Glycols/chemistry
- Zebrafish
- PubMed
- 37590033 Full text @ Adv. Healthc. Mater.
Citation
Canato, E., Grigoletto, A., Zanotto, I., Tedeschini, T., Campara, B., Quaglio, G., Toffoli, G., Mandracchia, D., Dinarello, A., Tiso, N., Argenton, F., Sayaf, K., Guido, M., Gabbia, D., De Martin, S., Pasut, G. (2023) Anti-HER2 Super Stealth Immunoliposomes for Targeted-Chemotherapy. Advanced Healthcare Materials. 12(29):e2301650.
Abstract
Liposomes play an important role in the field of drug delivery by virtue of their biocompatibility and versatility as carriers. Stealth liposomes, obtained by surface decoration with hydrophilic polyethylene glycol (PEG) molecules, represented an important turning point in liposome technology, leading to significant improvements in the pharmacokinetic profile compared to naked liposomes. Nevertheless, the generation of effective targeted liposomes - a central issue for cancer therapy - has faced several difficulties and clinical phase failures. Active targeting remains a challenge for liposomes. In this direction, we designed a new Super Stealth Immunoliposomes (SSIL2) composed of a PEG-bi-phospholipids derivative that stabilizes the polymer shielding over the liposomes. Furthermore, its counterpart, conjugated to the fragment antigen-binding of trastuzumab (Fab'TRZ -PEG-bi-phospholipids), is firmly anchored on the liposomes surface and correctly orients outward the targeting moiety. Throughout this study, the performances of SSIL2 are evaluated and compared to classic stealth liposomes and stealth immunoliposomes in vitro in a panel of cell lines and in vivo studies in zebrafish larvae and rodent models. Overall, SSIL2 shows superior in vitro and in vivo outcomes, both in terms of safety and anticancer efficacy, thus representing a step forward in targeted cancer therapy, and valuable for future development. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping