PUBLICATION
Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells
- Authors
- Hagio, H., Koyama, W., Hosaka, S., Song, A.D., Narantsatsral, J., Matsuda, K., Sugihara, T., Shimizu, T., Koyanagi, M., Terakita, A., Hibi, M.
- ID
- ZDB-PUB-230818-44
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Hibi, Masahiko, Shimizu, Takashi
- Keywords
- bistable rhodopsin, cardiac contraction, cell biology, g protein-coupled receptor, g protein-coupled rhodopsin, locomotion, neuroscience, optogenetics, zebrafish
- MeSH Terms
-
- Animals
- Myocytes, Cardiac*
- Neurons
- Optogenetics
- Potassium Channels, Inwardly Rectifying*
- Rhodopsin
- Zebrafish
- PubMed
- 37589544 Full text @ Elife
Citation
Hagio, H., Koyama, W., Hosaka, S., Song, A.D., Narantsatsral, J., Matsuda, K., Sugihara, T., Shimizu, T., Koyanagi, M., Terakita, A., Hibi, M. (2023) Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells. eLIFE. 12:.
Abstract
G-protein-coupled receptors (GPCRs) transmit signals into cells depending on the G protein type. To analyze the functions of GPCR signaling, we assessed the effectiveness of animal G-protein-coupled bistable rhodopsins that can be controlled into active and inactive states by light application using zebrafish. We expressed Gq- and Gi/o-coupled bistable rhodopsins in hindbrain reticulospinal V2a neurons, which are involved in locomotion, or in cardiomyocytes. Light stimulation of the reticulospinal V2a neurons expressing Gq-coupled spider Rh1 resulted in an increase in the intracellular Ca2+ level and evoked swimming behavior. Light stimulation of cardiomyocytes expressing the Gi/o-coupled mosquito Opn3, pufferfish TMT opsin, or lamprey parapinopsin induced cardiac arrest, and the effect was suppressed by treatment with pertussis toxin or barium, suggesting that Gi/o-dependent regulation of inward-rectifier K+ channels controls cardiac function. These data indicate that these rhodopsins are useful for optogenetic control of GPCR-mediated signaling in zebrafish neurons and cardiomyocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping